Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
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This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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An enzymatic amplification Flow Injection Analysis (FIA) system for the sensitive determination of phenol
B. Fuhrmann,Uwe Spohn +1 more
TL;DR: A highly sensitive, enzymatic FIA procedure was developed to determine phenol fluorimetrically by the automatic FIA procedure with high operational stability and the chemoenzymatic substrate recycling enhances the sensitivity of the detection of phenol and catechol.
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Nephroprotective and Anti-Inflammatory Effects of Pistacia atlantica Leaf Hydroethanolic Extract Against Gentamicin-Induced Nephrotoxicity in Rats.
Esfandiar Heidarian,Effat Jafari-Dehkordi,Parisa Valipour,Keihan Ghatreh-Samani,Leila Ashrafi-Eshkaftaki +4 more
TL;DR: The present study suggests that P. atlantica leaf hydroethanolic extract has protective effects against gentamicin-induced nephrotoxicity in rats, and histopathological studies show that in lymphocyte infiltration, remarkable reduction was observed in P.Atlanticas leaf hydro Ethanol extract-treated groups, compared with the untreated group.
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Nutritional composition of three estuarine bivalve mussels, Perna viridis, Donax cuneatus and Meretrix meretrix.
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On the potential increase of the oxidative stress status in patients with abdominal aortic aneurysm
Joël Pincemail,Jean Defraigne,Jean-Paul Cheramy-Bien,Nadia Dardenne,Anne-Françoise Donneau,Adelin Albert,Nicos Labropoulos,Natzi Sakalihasan +7 more
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References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.