Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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Antioxidant nutrient supplementation and brachial reactivity in patients with coronary artery disease
TL;DR: It is concluded that antioxidant supplementation did not significantly alter brachial reactivity, despite significantly increased plasma levels of antioxidants and improved lag phase, and these data should be confirmed in larger-scale trials and examined in studies evaluating individual dietary antioxidant supplementation.
Journal ArticleDOI
Role of selenium and vitamin C in mitigating oxidative stress induced by fenitrothion in rat liver.
TL;DR: Coadministration of FNT with Se and Vit C exhibited hepatoprotective role confirmed by reduction of oxidative stress levels and restoration in the values of examined parameters, showed a reduction in absolute and relative liver weight after FNT administration.
Journal ArticleDOI
Hazelnut oil administration reduces aortic cholesterol accumulation and lipid peroxides in the plasma, liver, and aorta of rabbits fed a high-cholesterol diet
Aydan Hatipoglu,Öznur Kanbagli,Jale Balkan,Mutlu Küçük,Ugur Cevikbas,Gülçin Aykaç-Toker,Hakan Berkkan,Müjdat Uysal +7 more
TL;DR: Hazelnut oil supplementation reduced oxidative stress and cholesterol accumulation in the aortas of rabbits fed an HC diet, without any decreasing effect on cholesterol levels in the plasma or liver.
Journal ArticleDOI
Antihistamine effects and complications of supplemental vitamin C
TL;DR: Chatterjee et al. as discussed by the authors examined whether 500 mg ascorbate daily, a level that could be obtained in a carefully planned diet, had an antihistamine effect and found that chronic administration of 2 g of Lascorbate per day has significantly lowered blood histamine levels by 40% in healthy adults; this reduction in histamine was inversely related to leukocyte chemotaxis, a measure of immune responsiveness.
Journal ArticleDOI
Changes in the reduction state of ascorbate and glutathione, protein oxidation and hydrolysis leading to the development of dehydration intolerance in Triticum aestivum L. seedlings
TL;DR: In this paper, the authors demonstrate that the trans- tion of wheat (Triticum aestivum L.) seedlings from dehydration tolerance to intolerance on the fifth day fol- lowing imbibition is associated with a disturbance in cel- lular redox homeostasis.
References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.