Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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Journal ArticleDOI
Oxidative stress in chronic hepatitis C: not just a feature of late stage disease.
Sanjiv K. Jain,Philip W. Pemberton,Alexander Smith,Raymond Mcmahon,Peter C. Burrows,Ali Aboutwerat,Thomas W. Warnes +6 more
TL;DR: Oxidant stress, as reflected in blood and urine by a wide range of pro- and antioxidant markers, is a significant feature of hepatitis C infection and may have a role in slowing disease progression to cirrhosis.
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Cadmium-induced lipid peroxidation and changes in antioxidant defense system in the rat testes: protective role of coenzyme Q(10) and vitamin E.
Branka I. Ognjanović,Snežana D. Marković,Nataša Z. Ðorđević,Ivana Trbojević,Andraš Š. Štajn,Zorica S. Saičić +5 more
TL;DR: The results suggest that both CoQ(10) and Vit E function as a potent antioxidant in protection of rats testes against the oxidative stress induced by Cd.
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Ameliorative potential of resveratrol on proinflammatory cytokines, hyperglycemia mediated oxidative stress, and pancreatic β-cell dysfunction in streptozotocin- nicotinamide-induced diabetic rats.
TL;DR: It is first‐time reported that the oral administration of resveratrol may effectively rescue β‐cells from oxidative damage without affecting their function and structural integrity and exhibits significant antidiabetic potential.
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Effect of the Lycium barbarum polysaccharides administration on blood lipid metabolism and oxidative stress of mice fed high-fat diet in vivo
TL;DR: This study revealed that HF diet induced oxidative stress and provided novel evidence regarding the link between high dietary fat and increased risk of degenerative diseases and showed a noticeable inhibition against lipid oxidation induced by free radicals caused by HF diet intake.
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Nutrient composition of kopyor coconuts (Cocos nucifera L.)
TL;DR: The relatively high contents of sucrose, glucose, fructose, citric and malic acids might contribute to the deliciousness of kopyor meat.
References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.