Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
More filters
Journal ArticleDOI
Protective Effect of Proanthocyanidins in Cadmium Induced Neurotoxicity in Mice
TL;DR: The data suggest that PACs plays a crucial role on neuroprotection in combating the cadmium induced oxidative neurotoxicity in mice brain by influencing the activation of AChE/Akt phosphorylation, antioxidant status, controlling the membrane damage (lipid peroxidation) and apoptotic protein caspase-3.
Journal ArticleDOI
Subacute effect of cigarette smoke exposure in rats: Protection by pot marigold (Calendula officinalis L.) extract
TL;DR: Investigation showed that administration of PM supplied relative protection against subacute CS-induced cell injury and glutathione peroxidase activities and β-carotene levels decreased in the CS group compared to control, however activities of these enzymes and concentration of vitamins were elevated by PM supplementation.
Journal ArticleDOI
The effect of different dietary levels of vitamin E and selenium on antioxidant status and immunological markers in serum of laying hens.
Zenon Zduńczyk,Aleksandra Drażbo,Jan Jankowski,Jerzy Juśkiewicz,Anna Czech,Zofia Antoszkiewicz +5 more
TL;DR: It is recommended that the levels of both supplements, Se and vitamin E, be increased in laying hen diets as they have a beneficial effect on the serum concentrations of retinol and alpha-tocopherol.
Journal ArticleDOI
Inhibitory effects of Cynodon dactylon L. on inflammation and oxidative stress in adjuvant treated rats.
TL;DR: Cynodon dactylon extract has a promising protective role against arthritis, as indicated by the significant attenuation in the inflammatory response, oxidative stress and ameliorated the arthritic changes to near normal conditions.
Journal ArticleDOI
Preventive effect of nerolidol on isoproterenol induced myocardial damage in Wistar rats: Evidences from biochemical and histopathological studies.
Loordhurani Asaikumar,Lakshmanan Vennila,Palaniyandi Akila,Subramanian Sivasangari,Kaliyamoorthi Kanimozhi,Vengatesan Premalatha,Ganapathi Sindhu +6 more
TL;DR: In the present study, ISO‐induced myocardial damage was indicated by the changes in body weight, heart weight and the cardiac and hepatic marker enzymes as well as activities of enzymatic antioxidants in erythrocytes and heart tissue.
References
More filters
Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.