Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
More filters
Journal ArticleDOI
Ameliorative effect of diosmin, a citrus flavonoid against streptozotocin-nicotinamide generated oxidative stress induced diabetic rats.
TL;DR: Results indicated that diosmin (DS) has potential ameliorative effects in addition to its antidiabetic effect in type 2 diabetic rats.
Journal ArticleDOI
Antihyperglycaemic and antioxidant effect of hyponidd, an ayurvedic herbomineral formulation in streptozotocin-induced diabetic rats
TL;DR: The results showed that hyponidd exhibits antihyperglycaemic and antioxidant activity in STZ‐induced diabetic rats.
Journal ArticleDOI
Antioxidant and anticancer efficacy of hesperidin in benzo(a)pyrene induced lung carcinogenesis in mice
Sattu Kamaraj,Gopalakrishnan Ramakrishnan,Pandi Anandakumar,Sundaram Jagan,Thiruvengadam Devaki +4 more
TL;DR: Findings substantiate the chemopreventive potential of hesperidin against chemically induced lung cancer in mice and show potent anticancer effect in lung cancer.
Journal ArticleDOI
Antihyperglycemic and antilipidperoxidative effects of Pongamia pinnata (Linn.) Pierre flowers in alloxan induced diabetic rats.
R. Punitha,Shanmugam Manoharan +1 more
TL;DR: The results suggested that the ethanolic extract of Pongamia pinnata (Linn.) Pierre (Leguminosae) flowers (PpEt) could be used as a safe alternative antihyperglycemic drug for diabetic patients.
Journal ArticleDOI
Chronic chromium exposure-induced changes in testicular histoarchitecture are associated with oxidative stress: study in a non-human primate (Macaca radiata Geoffroy)
M. Michael Aruldhas,S. Subramanian,P. Sekar,G. Vengatesh,Gowri Chandrahasan,P. Govindarajulu,Mohammad Abdulkader Akbarsha +6 more
TL;DR: CrVI disrupts spermatogenesis by inducing free radical toxicity, and supplementation of antioxidant vitamins may be beneficial to the affected subjects.
References
More filters
Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.