Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
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This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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Journal ArticleDOI
Reduced mononuclear leukocyte ascorbic acid content in adults with insulin-dependent diabetes mellitus consuming adequate dietary vitamin C.
John J. Cunningham,Sarah L. Ellis,Sarah L. Ellis,Karen L. McVeigh,Karen L. McVeigh,Robin E. Levine,Robin E. Levine,Jorge Calles-Escandon,Jorge Calles-Escandon +8 more
TL;DR: The measurement of the AA content of mononuclear leukocytes (MN-AA) suggests an impaired tissue AA storage in adults with IDDM and supports the theory that intracellular scurvy contributes to the chronic degenerative complications of the disease.
Journal ArticleDOI
Compromised antioxidant status associated with ascites in broilers.
TL;DR: There were no differences in tissue ascorbate, tocopherol, and GSH between the control and NASC groups with the exception of hepatic toCopherol at 7 wk, which was higher in NASC birds than in ASC and control birds.
Journal ArticleDOI
Plasma Vitamin C Is Inversely Related to Body Mass Index and Waist Circumference but Not to Plasma Adiponectin in Nonsmoking Adults
TL;DR: Plasma vitamin C was inversely related to markers of adiposity, particularly in women, but vitamin C supplementation did not influence the circulating concentration of adiponectin.
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Lipid peroxidation in focal cerebral ischemia.
TL;DR: The results suggest functional and durational differences between antioxidants and lipid peroxidation in this ischemic model and suggest the possible involvement of the xanthine-xanthine oxidase system in initiating free radical reactions in cerebral ischemia.
Journal ArticleDOI
Eating behaviors, nutritional status, and menstrual function in elite female adolescent volleyball players.
TL;DR: The results indicate that elite adolescent volleyball players are at risk for menstrual dysfunction and have energy and nutrient intakes that place them atrisk for nutritional deficiencies and compromised performance.
References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.