Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
Reads0
Chats0
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
More filters
Journal ArticleDOI
Evidence for early oxidative stress in acute pancreatitis : clues for correction
Joan M. Braganza,Patrick Scott,D Bilton,David Schofield,Christopher Chaloner,Neil Shiel,Linda P. Hunt,Teodoro Bottiglieri +7 more
TL;DR: It is concluded that oxidative stress has pervaded the vascular compartment by the time of admission in patients with acute pancreatitis, and that blood micronutrient antioxidant profiles at this stage are consistent not only with compromised intracellular capacity to synthesize/refurbish glutathione, but also vulnerability of intra- and extracellular lipid targets.
Journal ArticleDOI
Antioxidant property of α-asarone against noise-stress-induced changes in different regions of rat brain
TL;DR: The data proved that antioxidant property of α-asarone against noise-stress induced changes in the rat brain, and more clinical studies are required to investigate effectiveness of the α-aserone in noisy environment in human subjects.
Journal ArticleDOI
Modulatory influence of dietary resveratrol during different phases of 1,2-dimethylhydrazine induced mucosal lipid-peroxidation, antioxidant status and aberrant crypt foci development in rat colon carcinogenesis.
TL;DR: It is indicated that resveratrol effectively inhibits DMH-induced ACF and colonic tumor development and modulation of lipid peroxidation markers and antioxidants status, which were paralleled with ACF suppression, as compared toDMH-alone treated rats.
Journal ArticleDOI
Protective effect of DL-α-lipoic acid on cyclophosphamide induced oxidative cardiac injury
Yenjerla Mythili,Periyasamy Thandavan Sudharsan,Elangovan Selvakumar,Palaninathan Varalakshmi +3 more
TL;DR: Cardiotoxicity, evident from increased activities of serum creatine phosphokinase, lactate dehydrogenase, aspartatetransaminase and alanine transaminase in CP administered rats, was reversed by LA treatment, highlighting the efficacy of LA as a cytoprotectant in CP induced cardiotoxicity.
Journal ArticleDOI
A diet rich in fat and poor in dietary fiber increases the in vitro formation of reactive oxygen species in human feces
TL;DR: It is confirmed that diets high in fat and meat and low in fiber markedly increase the potential for hydroxyl radical formation in the feces, which in turn may contribute to an enhanced risk of colorectal cancer.
References
More filters
Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.