Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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Effect of Mucuna pruriens on oxidative stress mediated damage in aged rat sperm.
TL;DR: Sperm damage in ageing was significantly reduced by quenching ROS, improving antioxidant defence system and mitochondrial function, and present observation indicates the antioxidant enhancing property, free radical quenched ability and spermatogenic efficacy of the M. pruriens.
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Zingerone induced caspase-dependent apoptosis in MCF-7 cells and prevents 7,12-dimethylbenz(a)anthracene-induced mammary carcinogenesis in experimental rats.
Hong-Yun Gan,Yaqing Zhang,Qingyun Zhou,Lierui Zheng,Xiao-Feng Xie,Vishnu Priya Veeraraghavan,Surapaneni Krishna Mohan +6 more
TL;DR: The anticancer effect of ZO toward DMBA‐induced mammary cancer in SD animals and Michigan cancer foundation‐7 mammarycancer cells is proposed and supported by the in vitro study.
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Effect of Terminalia arjuna stem bark on antioxidant status in liver and kidney of alloxan diabetic rats.
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TL;DR: The result indicates that the extract of Terminalia arjuna stem bark exhibit the antioxidant activity through correction of oxidative stress and validates the traditional use of this plant in diabetic animals.
Modifications of spectrophotometric methods for antioxidative vitamins determination convenient in analytic practice
TL;DR: In this paper, the authors have elaborated modifications of valuable spectrophotometric methods for de-termination each of those vitamins, which originally could not be applied for laboratory practice from various regards.
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Effect of curcumin on selenite-induced cataractogenesis in Wistar rat pups.
TL;DR: The presence of oxidative stress in selenite cataract development and its prevention byCurcumin support the possibility that the natural consumption of curcumin in food can help prevent the onset of senile cataracts.
References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.