Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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Journal ArticleDOI
Fluorometric determination of total vitamin C in whole blood by high-performance liquid chromatography with pre-column derivatization
TL;DR: Investigations showed that total vitamin C in whole blood is stable for eight days at -20 degrees C, provided ethyleneglycol-bis-(beta-amino-ethyl ether)-N,N, N',N',N'-tetraacetic acid and glutathione are immediately added to the blood sample.
Journal ArticleDOI
Metabolic engineering of an alternative pathway for ascorbic acid biosynthesis in plants
Ashok Jain,Craig L. Nessler +1 more
TL;DR: Transgenic tobacco and lettuce plants expressing a rat cDNA encoding L-gulono-γ-lactone oxidase accumulated up to seven times more ascorbic acid than untransformed plants demonstrate that basal levels of ascorBic acid in plants can be significantly increased by expressing a single gene from the animal pathway.
Journal ArticleDOI
Oxidative Processes in Muscle Systems and Fresh Meat: Sources, Markers, and Remedies.
TL;DR: This review outlines the potential use of 1 group of biomarkers, the isoprostanes, in the context of complex biochemical reactions relating to oxidative processes that take place in the biological systems of live animals and subsequently in meat (in vitro).
Journal ArticleDOI
Milk from Mothers of Both Premature and Full-Term Infants Provides Better Antioxidant Protection than Does Infant Formula
TL;DR: It is concluded that human milk has better antioxidant protection than do formulas, perhaps because of the higher iron content of formulas.
Journal ArticleDOI
Antioxidant effect of tetrahydrocurcumin in streptozotocin-nicotinamide induced diabetic rats.
TL;DR: Results of the present study indicated that THC showed antioxidant effect in addition to its antidiabetic effect in type 2 diabetic rats, suggesting its role in protection against lipid peroxidation induced membrane damage.
References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.