Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
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This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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Journal ArticleDOI
Comparison of respiratory syncytial virus humoral immunity and response to infection in young and elderly adults.
Ann R. Falsey,Edward E. Walsh,R. John Looney,John E. Kolassa,Maria A. Formica,Mary C. Criddle,William J. Hall +6 more
TL;DR: The immune response of older persons to RSV infection was as vigorous as the younger subjects, with 79% having a ≥fourfold rise in MNA titers compared to 64% in the young, suggesting that the severe clinical manifestations of RSV in the elderly are not due to a significant defect in humoral immunity.
Journal ArticleDOI
Ascorbic acid concentrations in dimethylnitrosamine-induced hepatic fibrosis in rats
TL;DR: The most probable reason for the decreased blood and liver ascorbic acid during DMN-induced hepatic fibrosis is the increased utilization of ascorBic acid for free radical scavenging in order to reduce the highly elevated oxidative stress.
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Remedial effect of DL-α-lipoic acid against adriamycin induced testicular lipid peroxidation
TL;DR: Treatment with lipoic acid, a “universal antioxidant” was investigated on ADR induced peroxidative damages in rat testis, proving it to be an effective cytoprotectant.
Journal ArticleDOI
Effect of Syzigium cumini in Plasma Antioxidants on Alloxan-Induced Diabetes in Rats
TL;DR: The antioxidant activity of an aqueous extract of the seeds of Syzigium cumini, an indigenous plant present in different parts of India, South-East Asia and Eastern Africa, toward experimental diabetes was evaluated and insulin restored all the parameters to their normal values.
Journal ArticleDOI
Flow-injection chemiluminometric determination of ascorbic acid based on its sensitized photooxidation
TL;DR: In this paper, a flow-injection configuration is proposed for the determination of ascorbic acid over a concentration range of 1 × 10−9 −3 × 10 −4 M with a throughput of 80 samples/h.
References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.