Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
More filters
Journal ArticleDOI
Functional muscle impairment in facioscapulohumeral muscular dystrophy is correlated with oxidative stress and mitochondrial dysfunction.
Ahmed Turki,Maurice Hayot,Gilles Carnac,Fabien Pillard,Emilie Passerieux,Sébastien Bommart,Eric Raynaud de Mauverger,Gérald Hugon,Joël Pincemail,Sylvia Pietri,Karen Lambert,Alexandra Belayew,Yegor S. Vassetzky,Raul Juntas Morales,Jacques Mercier,Dalila Laoudj-Chenivesse +15 more
TL;DR: The data characterized oxidative stress in patients with FSHD and demonstrated a correlation with their peripheral skeletal muscle dysfunction, suggesting that antioxidants that might modulate or delay oxidative insult may be useful in maintaining F SHD muscle functions.
Journal ArticleDOI
Leukocyte lipid peroxidation, superoxide dismutase, glutathione peroxidase and serum and leukocyte vitamin C levels of patients with type II diabetes mellitus
TL;DR: Leukocytes of diabetics are affected by oxidative stress which might be a reason for decreased microbicidal activity and there was no correlation between the above parameters and HbA1c and glucose levels.
Journal ArticleDOI
Implications for degenerative disorders: antioxidative activity, total phenols, flavonoids, ascorbic acid, beta-carotene and beta-tocopherol in Aloe vera
TL;DR: In this article, the aqueous extract from Aloe vera leaves contained naturally occuring antioxidant components, including total phenols, flavonoids, ascorbic acid, β-carotene and α-tocopherol.
Journal ArticleDOI
Antihyperglycemic and antioxidant effects of a flavanone, naringenin, in streptozotocin–nicotinamide-induced experimental diabetic rats
Thangaraj Annadurai,Arumugam Ramachandran Muralidharan,T. Joseph,M. J. Hsu,Philip A. Thomas,P. Geraldine +5 more
TL;DR: It is suggested that naringenin exhibits antihyperglycemic and antioxidant effects in experimental diabetic rats, similar to those observed in glyclazide-treated animals.
Journal ArticleDOI
Modulatory effects of Aloe vera leaf gel extract on oxidative stress in rats treated with streptozotocin.
TL;DR: This study confirms the ethnopharmacological use of Aloe vera in ameliorating the oxidative stress found in diabetes and appears to be more effective than glibenclamide in controlling oxidative stress.
References
More filters
Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.