Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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Journal ArticleDOI
Lipoic acid ameliorates adriamycin-induced testicular mitochondriopathy.
TL;DR: The effect of lipoic acid (LA), a universal antioxidant was investigated on ADR-induced testicular toxicity in rats, thereby proving to be an effective cytoprotectant.
Journal ArticleDOI
Capsaicin modulates pulmonary antioxidant defense system during benzo(a)pyrene-induced lung cancer in Swiss albino mice.
Pandi Anandakumar,Sattu Kamaraj,Sundaram Jagan,Gopalakrishnan Ramakrishnan,Radhakrishnan Vinodhkumar,Thiruvengadam Devaki +5 more
TL;DR: The effect of a pungent ingredient of red pepper, capsaicin, on oxidative stress induced changes in the antioxidant defense system by benzo(a)pyrene in the lungs of mice was studied, highlighting the antioxidant potential of Capsaicin in mitigating the oxidative stress mediated damage produced during benzo('a')pyrene‐induced lung cancer.
Journal ArticleDOI
Serum and Red Blood Cell Antioxidant Status in Patients with Bronchial Asthma
Hüseyin Vural,Kürşat Uzun +1 more
TL;DR: The results suggest that reactive oxygen species may be a contributing factor in patients with asthma, causing changes in serum vitamin C, ceruloplasmin and erythrocyte glutathione levels.
Journal ArticleDOI
The pathway of L-ascorbic acid biosynthesis in the colourless microalga Prototheca moriformis
TL;DR: A biosynthetic pathway from Glc to AA is proposed in which the epimerase is the rate-limiting activity in AA synthesis, based on data from mutant strain UV77-247 of Prototheca moriformis Kruger, which synthesized l-ascorbic acid with approximately three-quarters of the label at the C-1 position and the remaining label at C-6 position.
Journal ArticleDOI
Evaluation of the anti-atherogenic potential of chrysin in Wistar rats
TL;DR: The results suggest that chrysin has anti-atherogenic potential in an experimental setting and Histopathological studies appeared to suggest the protective effect of chrys in on the hepatic tissue and aorta of atherosclerotic rats.
References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.