Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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Aroclor 1254 impairs spermatogenesis and induces oxidative stress in rat testicular mitochondria.
TL;DR: A1254 impairs spermatogenesis as evidenced, at least partly, by induction of oxidative stress in testicular mitochondria in a dose-related pattern.
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Antioxidant potentials and ajmalicine accumulation in Catharanthus roseus after treatment with giberellic acid.
C. Abdul Jaleel,Ragupathi Gopi,P. Manivannan,B. Sankar,A. Kishorekumar,Rajaram Panneerselvam +5 more
TL;DR: Preliminary results suggest that, the application of GA(3) may be a useful tool to increase the antioxidant potential and alkaloid production in medicinal plants like C. roseus.
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Effect of Terminalia chebula aqueous extract on oxidative stress and antioxidant status in the liver and kidney of young and aged rats
TL;DR: It is demonstrated that aqueous extract of T. cheubla inhibits the development of age‐induced damages by protecting against oxidative stress and enhanced antioxidant status in the liver and kidney of aged rats.
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Seasonal variations in plasma micronutrients and antioxidants.
Robert V. Cooney,Adrian A. Franke,Jean H. Hankin,Laurie J. Custer,Lynne R. Wilkens,Patricia J. Harwood,Loic Le Marchand +6 more
TL;DR: Assessment by a diet history of food and supplement intake of micronutrients and phytochemicals for 1 year showed good agreement with 1-year mean plasma levels for most carotenoids, vitamin C, and alpha-tocopherol.
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Effect of different contraceptive methods on the oxidative stress status in women aged 40–48 years from the ELAN study in the province of Liège, Belgium
Joël Pincemail,Sophie Vanbelle,Ulysse Gaspard,Georges Collette,Jeanine Haleng,Jean-Paul Cheramy-Bien,Corinne Charlier,Jean-Paul Chapelle,Didier Giet,Adelin Albert,Raymond Limet,Jean-Olivier Defraigne +11 more
TL;DR: The intake of OC significantly increases the lipid peroxidation in women aged 40-48 years, which may represent a potential cardiovascular risk factor for these women.
References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.