Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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Chronic depletion of glutathione (GSH) and minimal modification of LDL in vivo: its prevention by glutathione mono ester (GME) therapy
Namakkal S. Rajasekaran,Srinivasan Sathyanarayanan,Srinivasan Sathyanarayanan,Niranjali Devaraj,Halagowder Devaraj +4 more
TL;DR: Increasing the cellular GSH levels may trigger beneficial effects against oxidative stress, and the minimal modification on LDL does not lead to any vascular damage in the dorsal aorta of the rats injected with BSO.
Journal ArticleDOI
Effect of vitamin A on the antioxidant system of the chick during early postnatal development.
TL;DR: It is concluded that the effect of vitamin A on development of the antioxidant system in the neonatal chick is dose-dependent and an excess ofitamin A can compromise the antioxidant defence system.
Journal ArticleDOI
Hepato-renal protective effects of hydroethanolic extract of Senna alata on enzymatic and nonenzymatic antioxidant systems in streptozotocin induced diabetic rats.
TL;DR: It was very clear that, Senna alata has helped to manage the oxidative tension in diabetic rats, which in turn may greatly support the hypoglycaemic potency of SennaAlata L.
Journal Article
Protective Effects of Hydroalcoholic Extract of Ferulago Angulata Against Gentamicin-induced Nephrotoxicity in Rats.
TL;DR: Ferulago angulate extract has protective effects against nephrotoxicity due to gentamicin, and was linked with a lower renal gene expression of tumor necrosis factor-α.
Journal ArticleDOI
Plant growth regulators induced changes in antioxidant potential and andrographolide content in Andrographis paniculata Wall.ex Nees
V. E. Anuradha,Cheruth Abdul Jaleel,Mohamed A. Salem,M. Gomathinayagam,Rajaram Panneerselvam +4 more
TL;DR: In this article, the effect of growth regulators on the andrographolide content and antioxidant potentials of Andrographis paniculata plants was investigated by means of foliar spray during morning hours.
References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.