Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
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This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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Hypolipidemic and antioxidant potency of heat processed turmeric and red pepper in experimental rats
TL;DR: In this article, the hypocholesterolemic and antioxidant potency of both raw and pressure-cooked turmeric and red pepper were evaluated in experimental rats rendered hypercholesterolem by feeding 0.5% cholesterol enriched diet and maintained for 8 weeks on 5% spice diet.
Journal Article
A clinical trial to evaluate the effect of vitamin C supplementation on in vitro mutagen sensitivity. The University of Texas M. D. Anderson Clinical Community Oncology Program Network.
Terri M. King,Zoltan Trizna,Xifeng Wu,Christopher I. Amos,Rebecca H. Fueger,John J. Fueger,Herbert A. Fritsche,T. C. Hsu,Rodger J. Winn,Margaret R. Spitz +9 more
TL;DR: The results of a randomized clinical trial to determine whether increasing levels of oral ascorbic acid could reduce the levels of mutagen sensitivity did not find a dose-response relationship between ascorBic acid intake and mutagenensitivity.
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Optimization, characterization and hepatoprotective effect of polysaccharides isolated from Stipa parviflora Desf. against CCl4 induced liver injury in rats using surface response methodology (RSM).
TL;DR: The SPP fraction isolated from S. parviflora showed significant hepatoprotective potential which could be related to active compounds present in this fraction, which was evaluated by estimating the levels of serum markers like ALT and AST levels.
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Taurine Regulates Mitochondrial Function During 7,12-Dimethyl Benz[a]anthracene Induced Experimental Mammary Carcinogenesis.
Manickam Kalappan Vanitha,Kalpana Deepa Priya,Kuppusamy Baskaran,Kuppusamy Periyasamy,Dhravidamani Saravanan,Ramachandran Venkateswari,Balasundaram Revathi Mani,Aruldass Ilakkia,Sundaramoorthy Selvaraj,Rajendran Menaka,Mahendran Geetha,Nadarajah Rashanthy,Pandi Anandakumar,Dhanapal Sakthisekaran +13 more
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References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.