Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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Journal ArticleDOI
Effects of N-acetyl cysteine on oxidative stress and TNF-α gene expression in diclofenac-induced hepatotoxicity in rats.
TL;DR: This study confirmed the protective effect of N-acetyl cysteine on diclofenac-induced hepatotoxicity in rats due to not only reduces liver inflammatory cells, TNF-α, serum MDA, and PC but also through increases liver vitamin C, catalase, and superoxide dismutase activities.
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Cardioprotective effect of tincture of Crataegus on isoproterenol-induced myocardial infarction in rats.
TL;DR: The results show that pretreatment with TCR may be useful in preventing the damage induced by isoproterenol in rat heart.
Journal ArticleDOI
A Combination of Melatonin and Alpha Lipoic Acid has Greater Cardioprotective Effect than Either of them Singly Against Cadmium-Induced Oxidative Damage
Raktim Mukherjee,Raktim Mukherjee,Sudeep Banerjee,Niraj Joshi,Prem Kumar Singh,Darshee Baxi,A. V. Ramachandran +6 more
TL;DR: It is demonstrated that ML + ALA co-administration effectively protected against Cd-induced cardiac oxidative damage.
Journal ArticleDOI
Effect of ascorbic acid on copper and cholesterol in adult cynomolgus monkeys fed a diet marginal in copper
D B Milne,S T Omaye,W H Amos +2 more
TL;DR: The data suggest that high levels of ascorbic acid supplementation may make dietary copper relatively unavailable for regulating cholesterol metabolism.
Journal ArticleDOI
Anti-oxidant status in embryonic, post-hatch and larval stages of Asian seabass (Lates calcarifer)
N. Kalaimani,N. Chakravarthy,R. Shanmugham,A. R. Thirunavukkarasu,Shankar Vinayakarao Alavandi,T.C. Santiago +5 more
TL;DR: All the three anti-oxidant enzymes, SOD, CAT and GPx, showed high activities during gastrulation, suggesting an increased metabolic rate during the period of embryonic development, and an increased need to detoxify lipid peroxides during larval development.
References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.