Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
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Journal ArticleDOI
Status of lipids, lipid peroxidation, and antioxidant systems with Vitamin C supplementation during aging in rats
TL;DR: The effect of ascorbic acid supplementation was determined in young and aged rats for 15, 30, and 60 days, where cholesterol, triglycerides, phospholipids, and lipid peroxidation were considerably high, whereas, antioxidants superoxide dismutase, catalase, glutathione peroxidase,glutathione, ascorBic acid, and α-tocopherol were low.
Journal ArticleDOI
Comparative effects of different triazole compounds on antioxidant metabolism of Solenostemon rotundifolius.
A. Kishorekumar,C. Abdul Jaleel,P. Manivannan,B. Sankar,R. Sridharan,P.V. Murali,Rajaram Panneerselvam +6 more
TL;DR: The application of triazoles may be a useful tool to increase the antioxidant production in S. rotundifolius and thereby make it an economical food crop.
Journal ArticleDOI
Influence of stage of lactation and year season on composition of mares' colostrum and milk and method and time of storage on vitamin C content in mares' milk.
Maria Markiewicz-Keszycka,Grażyna Czyżak-Runowska,Jacek Wójtowski,Artur Jóźwik,Radosław Pankiewicz,Bogusława Łęska,J. Krzyzewski,Nina Strzałkowska,Joanna Marchewka,Emilia Bagnicka +9 more
TL;DR: Investigating the impact of stage of lactation and season on chemical composition, somatic cell count and some physicochemical parameters of mare' colostrum and milk and developing a method for the determination of vitamin C in mares' milk confirmed that mare's milk is a raw material with a unique chemical composition different from that produced by other farm animals.
Journal ArticleDOI
Microbial, nutritional, and organoleptic quality of pomegranate juice following high-pressure homogenization and low-temperature pasteurization.
O. Benjamin,Dani Gamrasni +1 more
TL;DR: The potential of HPH as alternative method for pasteurization on a highly healthy fruit like pomegranate is shown, and the advantages in HPH process is described through comprehensive analysis for nutritional, microbial, and sensorial quality.
Journal ArticleDOI
Response of Lingual Ascorbic Acid Test and Salivary Ascorbate Levels to Changes in Ascorbic Acid Intake
TL;DR: It was concluded that lingual ascorbic acid test values and salivary asCorbate levels are not related to changes in ascorBic acid intake and are not consistent with plasma or leukocyte ascor bate concentrations.
References
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Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.