Book ChapterDOI
Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
Reads0
Chats0
TLDR
This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.Abstract:
Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.read more
Citations
More filters
Journal ArticleDOI
Effect of vitamin E and taurine treatment on lipid peroxidation and antioxidant defense in perchloroethylene-induced cytotoxicity in mice
TL;DR: Vitamin E and taurine supplemented mice showed a marked reversal of these metabolic changes related to cellular damage caused by PER, suggesting that PER induced cellular damage may be associated with lipid peroxidation and that can be effectively prevented by both vitamin E andTaurine.
Journal ArticleDOI
Ameliorative Effect of Epigallocatechin Gallate on Cardiac Hypertrophy and Fibrosis in Aged Rats.
TL;DR: It is evident that EGCG may potentially inhibit aging-induced cardiac hypertrophy, fibrosis, and apoptosis, thereby preserving cardiac function and the proposed mechanism would be inhibition of reactive oxygen species–dependent activation of TGF&bgr;1, TNF&agr;, and NF-&kgr;B signaling pathway.
Journal Article
Increased plasma level of cholesterol-5 beta,6 beta-epoxide in endometrial cancer patients.
TL;DR: A possible role for cholesterol-5 beta,6 beta-epoxide as a biomarker of endometrial cancer risk is suggested and further studies are warranted to investigate the possible association between the oxidation products of cholesterol and endometricrial cancer and to determine the interactive role of antioxidants.
Research Communications Lipid peroxidation in liver of vitamin B-6 deficient rats
TL;DR: Liver homogenate of vitamin B-6 deficient rats showed enhanced susceptibility to lipid peroxidation upon incubation with NADPH or ascorbate or t-butyl hydroperoxide, which may be an indirect secondary effect of the general disruption of metabolism associated with vitamin B -6 deficiency.
Journal ArticleDOI
The comparison of antioxidative/oxidative profile in colostrum, milk and blood of early post-partum cows during their first and second lactation.
E. Albera,Marta Kankofer +1 more
TL;DR: Results which showed dynamic changes of antioxidative/oxidative profile not only in blood but also in colostrum/milk within examined period of time suggested appropriate answer of organism to current challenge.
References
More filters
Journal ArticleDOI
The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid
Joseph H. Roe,Carl A. Kuether +1 more
Journal ArticleDOI
Distribution of ascorbic acid, metabolites and analogues in man and animals.
TL;DR: The availability of labeled AA, metabolites, and analogues has made it possible to follow up the appearance of these compounds or mctabolites thereof in various tissues of animals by means of dissecting the animals with subsequent determination of the radioactive material accumulated by the tissues or by Means of whole-body autoradiography.
Journal ArticleDOI
A rapid micromethod for the determination of ascorbic acid in plasma and tissues.
TL;DR: A rapid simple micromethod for the determination of l -ascorbic acid in plasma and other biological tissues using orthophosphoric acid and ferric iron is presented and can be used to accurately determine 0.1 μg of the vitamin in samples of plasma andOther biological tissues.