The centrosome cycle: Centriole biogenesis, duplication and inherent asymmetries
Erich A. Nigg,Tim Stearns +1 more
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The spatial aspects of the centrosome duplication cycle, the mechanism of centriole assembly and the possible consequences of the inherent asymmetry of Centrosomes and centrosomes are discussed.Abstract:
Centrosomes are microtubule-organizing centres of animal cells. They influence the morphology of the microtubule cytoskeleton, function as the base for the primary cilium and serve as a nexus for important signalling pathways. At the core of a typical centrosome are two cylindrical microtubule-based structures termed centrioles, which recruit a matrix of associated pericentriolar material. Cells begin the cell cycle with exactly one centrosome, and the duplication of centrioles is constrained such that it occurs only once per cell cycle and at a specific site in the cell. As a result of this duplication mechanism, the two centrioles differ in age and maturity, and thus have different functions; for example, the older of the two centrioles can initiate the formation of a ciliary axoneme. We discuss spatial aspects of the centrosome duplication cycle, the mechanism of centriole assembly and the possible consequences of the inherent asymmetry of centrioles and centrosomes.read more
Citations
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Book ChapterDOI
Time-lapse imaging of primary cilium behavior with physiological expression of fluorescent ciliary proteins.
TL;DR: A detailed description of the methods for time-lapse imaging of primary cilium, from the generation of cell lines that stably express fluorescent protein-labeled cilia-localized proteins at the physiological level to image analysis, including quantification through image acquisition, is presented in this article .
Dissertation
Regulation and function of Rootletin - a gene differentially expressed in Drosophila sensory neurons
TL;DR: It is shown that CG6129 knock-down causes severe disruption of the chordotonal organs function without any obvious change in the structure of the cilium, other than the lack of ciliary rootlet, which further highlights the difference between the two types of ciliated sensory organs.
Posted ContentDOI
The Golgi checkpoint: Golgi unlinking during G2 is required for correct spindle formation and cytokinesis
TL;DR: In this article , a strategy to accumulate cells in G2 with an intact Golgi ribbon and then induce entry into mitosis was devised to understand the physiological significance of the pre-mitotic Golgi unlinking.
References
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The primary cilium: a signalling centre during vertebrate development
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Journal ArticleDOI
A Mechanism Linking Extra Centrosomes to Chromosomal Instability
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Journal ArticleDOI
Proteomic characterization of the human centrosome by protein correlation profiling
Jens S. Andersen,Christopher J. Wilkinson,Thibault Mayor,Thibault Mayor,Peter Mortensen,Erich A. Nigg,Matthias Mann +6 more
TL;DR: A mass-spectrometry-based proteomic analysis of human centrosomes in the interphase of the cell cycle by quantitatively profiling hundreds of proteins across several centrifugation fractions identified and validated 23 novel components and identified 41 likely candidates as well as the vast majority of the known centrosomal proteins in a large background of nonspecific proteins.
Journal ArticleDOI
Hippo signaling: growth control and beyond
Georg Halder,Randy L. Johnson +1 more
TL;DR: Recently discovered mechanisms that contribute to the dynamic regulation of Hippo signaling during Drosophila and vertebrate development are reviewed and exciting new insights are provided into the elusive mechanisms that regulate organ growth and regeneration.
Journal ArticleDOI
Cleavage of Cohesin by the CD Clan Protease Separin Triggers Anaphase in Yeast
TL;DR: It is shown here that separin is a cysteine protease related to caspases that alone can cleave Sccl in vitro and depends on a conserved protein called separin for sister chromatid separation.