The centrosome cycle: Centriole biogenesis, duplication and inherent asymmetries
Erich A. Nigg,Tim Stearns +1 more
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The spatial aspects of the centrosome duplication cycle, the mechanism of centriole assembly and the possible consequences of the inherent asymmetry of Centrosomes and centrosomes are discussed.Abstract:
Centrosomes are microtubule-organizing centres of animal cells. They influence the morphology of the microtubule cytoskeleton, function as the base for the primary cilium and serve as a nexus for important signalling pathways. At the core of a typical centrosome are two cylindrical microtubule-based structures termed centrioles, which recruit a matrix of associated pericentriolar material. Cells begin the cell cycle with exactly one centrosome, and the duplication of centrioles is constrained such that it occurs only once per cell cycle and at a specific site in the cell. As a result of this duplication mechanism, the two centrioles differ in age and maturity, and thus have different functions; for example, the older of the two centrioles can initiate the formation of a ciliary axoneme. We discuss spatial aspects of the centrosome duplication cycle, the mechanism of centriole assembly and the possible consequences of the inherent asymmetry of centrioles and centrosomes.read more
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Journal ArticleDOI
Procentriole assembly without centriole disengagement - a paradox of male gametogenesis.
TL;DR: It is provided compelling evidence that this general rule is overridden in insect gametogenesis, when distinct procentrioles are generated during prophase of the first meiosis while parent centriole are still engaged.
Journal ArticleDOI
Biomechanical control of meiotic chromosomal bouquet and germ cell morphogenesis by the zygotene cilium
Avishag Mytlis,Vineet Kumar,Qiu Tao,Rachael Deis,Neta Hart,Karine Levy,Markus Masek,Amal Shawahny,Adam Ahmad,Hagai Eitan,F. B. Nather,Shai Adar-Levor,Ramon Y. Birnbaum,Natalie Elia,Ruxandra Bachmann-Gagescu,Sudipto Roy,Yaniv M. Elkouby +16 more
TL;DR: It is demonstrated that the zygotene cilium is essential for chromosomal bouquet and synaptonemal complex formation, germ cell morphogenesis, ovarian development and fertility, and proposed a cellular paradigm that cilia can control chromosomal dynamics.
Book ChapterDOI
Centrosome Isolation and Analysis by Mass Spectrometry-Based Proteomics
TL;DR: Methods to isolate centrosomes from human cells and strategies to selectively identify and study the properties of the associated proteins using quantitative mass spectrometry-based proteomics are described.
Journal ArticleDOI
SASS6 overexpression is associated with mitotic chromosomal abnormalities and a poor prognosis in patients with colorectal cancer.
Kazuya Shinmura,Hisami Kato,Yuichi Kawanishi,Kiyoko Nagura,Takaharu Kamo,Yusuke Okubo,Yusuke Inoue,Nobuya Kurabe,Chunping Du,Moriya Iwaizumi,Kiyotaka Kurachi,Toshio Nakamura,Haruhiko Sugimura +12 more
TL;DR: The present findings revealed that the upregulation of SASS6 expression is involved in the pathogenesis of CRC and is associated with a poor prognosis among patients with colon cancer, and suggest that SASS 6 upregulation is a genetic abnormality relatively common in human cancer.
Journal ArticleDOI
Spatio-temporal regulation of RAG2 following genotoxic stress
William H. Rodgers,Jennifer N. Byrum,Hem Sapkota,Negar S. Rahman,Robert C. Cail,Shuying Zhao,David G. Schatz,Karla K. Rodgers +7 more
TL;DR: It is shown that formation of DSBs by treating cells with DNA damaging agents causes export of nuclear RAG2 from the nucleus to centrosomes, suggesting a novel mechanism for modulating cellular responses to D SBs in developing lymphocytes.
References
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Journal ArticleDOI
The primary cilium: a signalling centre during vertebrate development
TL;DR: The connections between cilia and developmental signalling have begun to clarify the basis of human diseases associated with ciliary dysfunction, and the cilium represents a nexus for signalling pathways during development.
Journal ArticleDOI
A Mechanism Linking Extra Centrosomes to Chromosomal Instability
TL;DR: It is demonstrated that cells with multiple centrosomes rarely undergo multipolar cell divisions, and the progeny of these divisions are typically inviable, and it is proposed that this mechanism may be a common underlying cause of CIN in human cancer.
Journal ArticleDOI
Proteomic characterization of the human centrosome by protein correlation profiling
Jens S. Andersen,Christopher J. Wilkinson,Thibault Mayor,Thibault Mayor,Peter Mortensen,Erich A. Nigg,Matthias Mann +6 more
TL;DR: A mass-spectrometry-based proteomic analysis of human centrosomes in the interphase of the cell cycle by quantitatively profiling hundreds of proteins across several centrifugation fractions identified and validated 23 novel components and identified 41 likely candidates as well as the vast majority of the known centrosomal proteins in a large background of nonspecific proteins.
Journal ArticleDOI
Hippo signaling: growth control and beyond
Georg Halder,Randy L. Johnson +1 more
TL;DR: Recently discovered mechanisms that contribute to the dynamic regulation of Hippo signaling during Drosophila and vertebrate development are reviewed and exciting new insights are provided into the elusive mechanisms that regulate organ growth and regeneration.
Journal ArticleDOI
Cleavage of Cohesin by the CD Clan Protease Separin Triggers Anaphase in Yeast
TL;DR: It is shown here that separin is a cysteine protease related to caspases that alone can cleave Sccl in vitro and depends on a conserved protein called separin for sister chromatid separation.