The centrosome cycle: Centriole biogenesis, duplication and inherent asymmetries
Erich A. Nigg,Tim Stearns +1 more
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TLDR
The spatial aspects of the centrosome duplication cycle, the mechanism of centriole assembly and the possible consequences of the inherent asymmetry of Centrosomes and centrosomes are discussed.Abstract:
Centrosomes are microtubule-organizing centres of animal cells. They influence the morphology of the microtubule cytoskeleton, function as the base for the primary cilium and serve as a nexus for important signalling pathways. At the core of a typical centrosome are two cylindrical microtubule-based structures termed centrioles, which recruit a matrix of associated pericentriolar material. Cells begin the cell cycle with exactly one centrosome, and the duplication of centrioles is constrained such that it occurs only once per cell cycle and at a specific site in the cell. As a result of this duplication mechanism, the two centrioles differ in age and maturity, and thus have different functions; for example, the older of the two centrioles can initiate the formation of a ciliary axoneme. We discuss spatial aspects of the centrosome duplication cycle, the mechanism of centriole assembly and the possible consequences of the inherent asymmetry of centrioles and centrosomes.read more
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Centrosomal MCM7 strengthens the Cep68-VHL interaction and excessive MCM7 leads to centrosome splitting resulting from increase in Cep68 ubiquitination and proteasomal degradation
Lingjun Kong,Huilong Yin,Li Yuan +2 more
TL;DR: Cep68 protein level needs to be fine-tuned in order to ensure that its direct interactors, such as the microcephaly protein Cep215 and PCNT, function properly.
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Babo1, formerly Vop1 and Cop1/2, is no eyespot photoreceptor but a basal body protein illuminating cell division in Volvox carteri.
TL;DR: The detailed subcellular localization of fluorescence-tagged Babo1 shows that it accumulates at the basal apparatus, and the unequal distribution of Babi1 in four-celled embryos could be an integral part of a geometrical system in early embryogenesis, which establishes the anterior-posterior polarity and influences the spatial arrangement of all embryonic structures and characteristics.
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CCDC84 Acetylation Oscillation Regulates Centrosome Duplication by Modulating HsSAS-6 Degradation.
TL;DR: CCDC84 constrains centriole number by modulating APC/CCdh1-mediated HsSAS-6 degradation and acetylation oscillates throughout the cell cycle, shedding new light on the function of (de)acetylation in centrioles number regulation as well as refine the established centrosome duplication model.
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Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.
TL;DR: It is found that SDAs are distinguishable from BF and that the protein NPHP5 is a novel SDA and BF component, which defines a novel role for N PHP5 in specifically regulating BF assembly, a process which is tightly coupled to primary ciliogenesis.
Journal ArticleDOI
Cep120 promotes microtubule formation through a unique tubulin binding C2 domain.
TL;DR: It is shown that Cep120 contains three consecutive C2 domains that are followed by a coiled-coil dimerization domain that binds to both tubulin and microtubules, and promotes microtubule formation.
References
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The primary cilium: a signalling centre during vertebrate development
TL;DR: The connections between cilia and developmental signalling have begun to clarify the basis of human diseases associated with ciliary dysfunction, and the cilium represents a nexus for signalling pathways during development.
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A Mechanism Linking Extra Centrosomes to Chromosomal Instability
TL;DR: It is demonstrated that cells with multiple centrosomes rarely undergo multipolar cell divisions, and the progeny of these divisions are typically inviable, and it is proposed that this mechanism may be a common underlying cause of CIN in human cancer.
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Proteomic characterization of the human centrosome by protein correlation profiling
Jens S. Andersen,Christopher J. Wilkinson,Thibault Mayor,Thibault Mayor,Peter Mortensen,Erich A. Nigg,Matthias Mann +6 more
TL;DR: A mass-spectrometry-based proteomic analysis of human centrosomes in the interphase of the cell cycle by quantitatively profiling hundreds of proteins across several centrifugation fractions identified and validated 23 novel components and identified 41 likely candidates as well as the vast majority of the known centrosomal proteins in a large background of nonspecific proteins.
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Hippo signaling: growth control and beyond
Georg Halder,Randy L. Johnson +1 more
TL;DR: Recently discovered mechanisms that contribute to the dynamic regulation of Hippo signaling during Drosophila and vertebrate development are reviewed and exciting new insights are provided into the elusive mechanisms that regulate organ growth and regeneration.
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Cleavage of Cohesin by the CD Clan Protease Separin Triggers Anaphase in Yeast
TL;DR: It is shown here that separin is a cysteine protease related to caspases that alone can cleave Sccl in vitro and depends on a conserved protein called separin for sister chromatid separation.