The centrosome cycle: Centriole biogenesis, duplication and inherent asymmetries
Erich A. Nigg,Tim Stearns +1 more
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TLDR
The spatial aspects of the centrosome duplication cycle, the mechanism of centriole assembly and the possible consequences of the inherent asymmetry of Centrosomes and centrosomes are discussed.Abstract:
Centrosomes are microtubule-organizing centres of animal cells. They influence the morphology of the microtubule cytoskeleton, function as the base for the primary cilium and serve as a nexus for important signalling pathways. At the core of a typical centrosome are two cylindrical microtubule-based structures termed centrioles, which recruit a matrix of associated pericentriolar material. Cells begin the cell cycle with exactly one centrosome, and the duplication of centrioles is constrained such that it occurs only once per cell cycle and at a specific site in the cell. As a result of this duplication mechanism, the two centrioles differ in age and maturity, and thus have different functions; for example, the older of the two centrioles can initiate the formation of a ciliary axoneme. We discuss spatial aspects of the centrosome duplication cycle, the mechanism of centriole assembly and the possible consequences of the inherent asymmetry of centrioles and centrosomes.read more
Citations
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ATX-2, the C. elegans Ortholog of Human Ataxin-2, Regulates Centrosome Size and Microtubule Dynamics
TL;DR: The data suggest that RNA-binding proteins play an active role in controlling MT dynamics and provide insight into the control of proper centrosome size and MT dynamics.
Journal ArticleDOI
Centrosomal cohesion deficits as cellular biomarker in lymphoblastoid cell lines from LRRK2 Parkinson's disease patients.
Belén Fernández,Antonio Ordóñez,Elena Fdez,Eugénie Mutez,Eugénie Mutez,Thomas Comptdaer,Thomas Comptdaer,Coline Leghay,Coline Leghay,Alexandre Kreisler,Alexandre Kreisler,Clémence Simonin,Clémence Simonin,Laurine Vandewynckel,Luc Defebvre,Alain Destée,Alain Destée,Séverine Bleuse,Jean-Marc Taymans,Jean-Marc Taymans,Marie-Christine Chartier-Harlin,Marie-Christine Chartier-Harlin,Sabine Hilfiker,Sabine Hilfiker +23 more
TL;DR: It is suggested that LCLs may be a valuable resource for LRRK2 research, and that determination of centrosomal cohesion deficits may assist in the stratification of a subset of sporadic PD patients.
Journal ArticleDOI
Par6γ is at the mother centriole and controls centrosomal protein composition through a Par6α-dependent pathway.
TL;DR: This study is the first to identify Par6&ggr; as a component of the mother centriole and to report a role of a mother centRIole protein in the regulation of centrosomal protein composition.
Journal ArticleDOI
Arl2- and Msps-dependent microtubule growth governs asymmetric division
Keng Chen,Chwee Tat Koe,Zhanyuan Benny Xing,Xiaolin Tian,Fabrizio Rossi,Cheng Wang,Quan Tang,Wenhui Zong,Wanjin Hong,Reshma Taneja,Fengwei Yu,Cayetano Gonzalez,Chunlai Wu,Sharyn A. Endow,Hongyan Wang +14 more
TL;DR: In this paper, it was shown that Drosophila ADP ribosylation factor like-2 (Arl2) and Msps, a known microtubule-binding protein, control cell polarity and spindle orientation of NBs.
Journal ArticleDOI
Inhibition of CPAP-tubulin interaction prevents proliferation of centrosome-amplified cancer cells.
Aruljothi Mariappan,Aruljothi Mariappan,Komal Soni,Kenji Schorpp,Fan Zhao,Fan Zhao,Amin Minakar,Xiangdong Zheng,Xiangdong Zheng,Sunit Mandad,Sunit Mandad,Iris Macheleidt,Anand Ramani,Tomáš Kubelka,Maciej Dawidowski,Maciej Dawidowski,Kristina Golfmann,Arpit Wason,Chunhua Yang,Judith Simons,Hans-Günther Schmalz,Anthony A. Hyman,Ritu Aneja,Roland T. Ullrich,Henning Urlaub,Henning Urlaub,Margarete Odenthal,Reinhardt Büttner,Haitao Li,Haitao Li,Michael Sattler,Kamyar Hadian,Jay Gopalakrishnan,Jay Gopalakrishnan +33 more
TL;DR: A vulnerability of cancer cells to activation of extra centrosomes is identified, which may serve as a global approach to target various tumors, including drug‐resistant cancers exhibiting high incidence of centrosome amplification.
References
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TL;DR: The connections between cilia and developmental signalling have begun to clarify the basis of human diseases associated with ciliary dysfunction, and the cilium represents a nexus for signalling pathways during development.
Journal ArticleDOI
A Mechanism Linking Extra Centrosomes to Chromosomal Instability
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Proteomic characterization of the human centrosome by protein correlation profiling
Jens S. Andersen,Christopher J. Wilkinson,Thibault Mayor,Thibault Mayor,Peter Mortensen,Erich A. Nigg,Matthias Mann +6 more
TL;DR: A mass-spectrometry-based proteomic analysis of human centrosomes in the interphase of the cell cycle by quantitatively profiling hundreds of proteins across several centrifugation fractions identified and validated 23 novel components and identified 41 likely candidates as well as the vast majority of the known centrosomal proteins in a large background of nonspecific proteins.
Journal ArticleDOI
Hippo signaling: growth control and beyond
Georg Halder,Randy L. Johnson +1 more
TL;DR: Recently discovered mechanisms that contribute to the dynamic regulation of Hippo signaling during Drosophila and vertebrate development are reviewed and exciting new insights are provided into the elusive mechanisms that regulate organ growth and regeneration.
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Cleavage of Cohesin by the CD Clan Protease Separin Triggers Anaphase in Yeast
TL;DR: It is shown here that separin is a cysteine protease related to caspases that alone can cleave Sccl in vitro and depends on a conserved protein called separin for sister chromatid separation.