The centrosome cycle: Centriole biogenesis, duplication and inherent asymmetries
Erich A. Nigg,Tim Stearns +1 more
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TLDR
The spatial aspects of the centrosome duplication cycle, the mechanism of centriole assembly and the possible consequences of the inherent asymmetry of Centrosomes and centrosomes are discussed.Abstract:
Centrosomes are microtubule-organizing centres of animal cells. They influence the morphology of the microtubule cytoskeleton, function as the base for the primary cilium and serve as a nexus for important signalling pathways. At the core of a typical centrosome are two cylindrical microtubule-based structures termed centrioles, which recruit a matrix of associated pericentriolar material. Cells begin the cell cycle with exactly one centrosome, and the duplication of centrioles is constrained such that it occurs only once per cell cycle and at a specific site in the cell. As a result of this duplication mechanism, the two centrioles differ in age and maturity, and thus have different functions; for example, the older of the two centrioles can initiate the formation of a ciliary axoneme. We discuss spatial aspects of the centrosome duplication cycle, the mechanism of centriole assembly and the possible consequences of the inherent asymmetry of centrioles and centrosomes.read more
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Positioning of Particles in Active Droplets
David Zwicker,David Zwicker,Johannes Baumgart,Stefanie Redemann,Stefanie Redemann,Thomas Müller-Reichert,Anthony A. Hyman,Frank Jülicher +7 more
TL;DR: This study can account for the observed positioning of centrioles and provides a general mechanism to control the position of particles within chemically active droplets.
Journal ArticleDOI
Structural characterization of procentrioles in Drosophila spermatids.
TL;DR: It is showed that a distinct procentriole is assembled close to the proximal region of the centrioles in early S13 spermatids at the onion stage, confirming previous reports of a proximal centriole‐like structure at the proximate end of the sperMatid centriol.
Journal ArticleDOI
14-3-3γ Prevents Centrosome Amplification and Neoplastic Progression
Amitabha Mukhopadhyay,Lalit Sehgal,Arunabha Bose,Anushree C. Gulvady,Parijat Senapati,Rahul Thorat,Srikanta Basu,Khyati Bhatt,Amol S. Hosing,Renu Balyan,Lalit Borde,Tapas K. Kundu,Sorab N. Dalal +12 more
TL;DR: The molecular basis of regulation ofCentrosome duplication by 14-3-3γ and inhibition of tumor growth by premature activation of the mitotic program and the disruption of centrosome clustering are uncovered.
Journal ArticleDOI
Cross-talk between CDK4/6 and SMYD2 regulates gene transcription, tubulin methylation, and ciliogenesis.
Linda Xiaoyan Li,Julie Xia Zhou,Xiaodong Wang,Hongbing Zhang,Peter C. Harris,James P. Calvet,Xiaogang Li +6 more
TL;DR: Targeting the up-regulated CDK4/6 and SMYD2 with inhibitors results in restoration of the primary cilium in tumor and cystic cells, which may normalize cilia-mediated extracellular signals that regulate growth, development, and cellular homeostasis.
Journal ArticleDOI
Integrity of the Pericentriolar Material Is Essential for Maintaining Centriole Association during M Phase
TL;DR: It is proposed that PCM disassembly is one of the driving forces for centriole separation during mitotic exit in cells whose cell cycle is arrested at M phase by STLC.
References
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The primary cilium: a signalling centre during vertebrate development
TL;DR: The connections between cilia and developmental signalling have begun to clarify the basis of human diseases associated with ciliary dysfunction, and the cilium represents a nexus for signalling pathways during development.
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A Mechanism Linking Extra Centrosomes to Chromosomal Instability
TL;DR: It is demonstrated that cells with multiple centrosomes rarely undergo multipolar cell divisions, and the progeny of these divisions are typically inviable, and it is proposed that this mechanism may be a common underlying cause of CIN in human cancer.
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Proteomic characterization of the human centrosome by protein correlation profiling
Jens S. Andersen,Christopher J. Wilkinson,Thibault Mayor,Thibault Mayor,Peter Mortensen,Erich A. Nigg,Matthias Mann +6 more
TL;DR: A mass-spectrometry-based proteomic analysis of human centrosomes in the interphase of the cell cycle by quantitatively profiling hundreds of proteins across several centrifugation fractions identified and validated 23 novel components and identified 41 likely candidates as well as the vast majority of the known centrosomal proteins in a large background of nonspecific proteins.
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Hippo signaling: growth control and beyond
Georg Halder,Randy L. Johnson +1 more
TL;DR: Recently discovered mechanisms that contribute to the dynamic regulation of Hippo signaling during Drosophila and vertebrate development are reviewed and exciting new insights are provided into the elusive mechanisms that regulate organ growth and regeneration.
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Cleavage of Cohesin by the CD Clan Protease Separin Triggers Anaphase in Yeast
TL;DR: It is shown here that separin is a cysteine protease related to caspases that alone can cleave Sccl in vitro and depends on a conserved protein called separin for sister chromatid separation.