Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
Reads0
Chats0
TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
More filters
Patent
Method for producing l-amino acid
TL;DR: In this paper, an L-amino acid is produced by culturing an E. coli which belongs to the Enterobacteriaceae family and which has been modified so that the acetyl-CoA synthetase activity is increased.
Journal ArticleDOI
The response of Aeromonas hydrophila to oxidative stress induced by exposure to hydrogen peroxide.
TL;DR: Data show that Aer.
Journal ArticleDOI
Detection of ozone-induced dna single strand breaks in murine bronchoalveolar lavage cells acutely exposed in vivo
TL;DR: The results of this study indicate that (1) O(3) induces DNA strand breaks in murine BAL cells at 0.25 and 0.5 ppm, as evidenced by statistically significant increases in the length of comet tails for O( 3)-exposed groups, and (2) the comet assay can be used to assess O (3)-induced SSBs for in vivo exposures.
Journal ArticleDOI
Biological effects of hydrogen peroxide administered intratumorally with or without irradiation in murine tumors.
T. Takaoka,Yuta Shibamoto,Masayuki Matsuo,Chikao Sugie,Taro Murai,Y. Ogawa,Akifumi Miyakawa,Yoshihiko Manabe,Takuhito Kondo,K. Nakajima,Dai Okazaki,Takahiro Tsuchiya +11 more
TL;DR: The biological effects and appropriate combination methods of irradiation and H2O2 in vivo and results of the present study could serve as a basis for evaluating results of various clinical studies on this treatment.
Journal ArticleDOI
Contributions of ferric uptake regulator Fur to the sensitivity and oxidative response of Acinetobacter baumannii to antibiotics.
TL;DR: In this article, the authors evaluated the contribution of fur to the sensitivity and oxidative response of A. baumannii AB5075 to colistin, gentamicin, rifampicin and tigecycline, and concluded that decreased capability of Δfur mutant to detoxify reactive oxygen species raised its susceptibility to antibiotics through Fenton chemistry.
References
More filters
Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.