Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Mechanisms of cell injury by activated oxygen species.
TL;DR: The hypothesis most consistent with all the available data suggests that O2- reduces a cellular source of ferric to ferrous iron, and the latter then reacts with H2O2 to produce a more potent oxidizing species, like the .OH or an equivalently reactive species.
Journal ArticleDOI
Measurement of Reactive Oxygen Species, Reactive Nitrogen Species, and Redox-Dependent Signaling in the Cardiovascular System: A Scientific Statement From the American Heart Association
Kathy K. Griendling,Rhian M. Touyz,Jay L. Zweier,Sergey Dikalov,William M. Chilian,Yeong-Renn Chen,David G. Harrison,Aruni Bhatnagar +7 more
TL;DR: Current methods for measuring reactive oxygen species and the secondary products they generate are reviewed and approaches for measuring redox status, oxidative stress, and the production of individual reactive oxygen and nitrogen species are suggested.
Journal ArticleDOI
Regulators of oxidative stress response genes in Escherichia coli and their functional conservation in bacteria
TL;DR: Despite the enormous genomic diversity of bacteria, oxidative stress response regulators in E. coli are functionally conserved in a wide range of bacterial groups, possibly reflecting positive selection of these regulators.
Journal ArticleDOI
Heat-induced formation of reactive oxygen species and 8-oxoguanine, a biomarker of damage to DNA
TL;DR: Findings imply that heat acts via ROS attack leading to oxidative damage to DNA, which implies that heat-mediated generation of reactive oxygen species (ROS) should occur.
Patent
High-fat diet.
Denis Breuille,Gayle Crozier-Willi,Guy Dutot,Paul-André Finot,Myriam Richelle,Claudia Roessle,Marco Turini +6 more
TL;DR: The use of a composition comprising at least one lipid which provides between 35% and 75% of the total energy of the composition, the lipid 25% comprising 70% by weight of total lipid of MCT (triglycerides average) chain fatty acids of n-6 and n-3 in a ratio between 2/1 and 7/1, for the manufacture of a medicament, a functional food or a nutritive product for the treatment or prevention of sepsis or inflammatory shock.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.