Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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The bactericidal action of peroxides; an E.P.R. spin-trapping study.
TL;DR: The inhibition of bactericidal action, by DMPO and two antioxidants, Vitamin C and Trolox C, indicates that radicals are the lethal species and evidence is presented which suggests that radical production is internal to the bacterial cell.
Journal ArticleDOI
Systems-level understanding of ethanol-induced stresses and adaptation in E. coli.
Huansheng Cao,Du Wei,Du Wei,Yuedong Yang,Yu Shang,Yu Shang,Gaoyang Li,Gaoyang Li,Yaoqi Zhou,Qin Ma,Ying Xu,Ying Xu,Ying Xu +12 more
TL;DR: A computational study of transcriptomic and genomic data of both ethanol-stressed and ethanol-adapted E. coli cells with computationally predicated ethanol-binding proteins and experimentally identified ethanol tolerance genes suggests that new insights into the energy and mass balance will inform design of more ethanol-tolerant strains.
Journal Article
Tamoxifen suppresses tumor promoter-induced hydrogen peroxide formation by human neutrophils.
TL;DR: It is reported that TAM (5 microM) totally inhibits hydrogen peroxide (H2O2) formation by 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-treated human neutrophils, and may suppress the dietary fat-induced HMU in the same manner at it does in TPA-induced neutrophil.
Journal ArticleDOI
Fluorescent probes for the detection of catalytic Fe(II) ion.
TL;DR: Current advances in the development of fluorescent and bioluminescent probes that can selectively detect catalytic Fe(II) together with their biological applications accelerate the understanding of iron‐mediated oxidative stress as well as iron biology.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.