Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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The Use of the single cell gel electrophoresis assay in detecting DNAsingle strand breaks in lung cells in vitro
TL;DR: Findings indicate that SCGE can be used to detectSSB in cultured lung cells, and has applicability for detecting SSB in lung cells recovered from in vivo and in vitro exposures to oxidants.
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Analysis of DNA Strand Breaks, Oxidized Bases, and Glutathione S-Transferase P1 in Human Colon Cells from Biopsies
Beatrice L. Pool-Zobel,S.L. Abrahamse,Andrew Collins,Wolfgang Kark,Roland Gugler,Daniela Oberreuther,Eberhard Günther Siegel,Sylvia Treptow-van Lishaut,Gerhard Rechkemmer +8 more
TL;DR: In this article, the authors determined background levels of DNA breaks or oxidized bases and of glutathione S -transferases (GSTs) as potential biomarkers of risk and chemoprevention, respectively.
Journal ArticleDOI
Powerful bacterial killing by buckwheat honeys is concentration-dependent, involves complete DNA degradation and requires hydrogen peroxide.
TL;DR: Honey of buckwheat origin exhibited powerful, concentration-dependent bactericidal effect and the killing and DNA degradation showed a cause-effect relationship, indicating that honey dose was critical for its bactericidal efficacy.
Journal ArticleDOI
IscR modulates catalase A (KatA) activity, peroxide resistance and full virulence of Pseudomonas aeruginosa PA14.
TL;DR: The results suggest that the requirement of IscR in P. aeruginosa is related to the proper activity of KatA, which is crucial for peroxide resistance and full virulence of this bacterium.
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Ferric iron increases ROS formation, modulates cell growth and enhances genotoxic damage by 4-hydroxynonenal in human colon tumor cells.
TL;DR: High concentrations of Fe-NTA caused cellular effects, which reflect a stress response, and resulted in formation of ROS, which enhanced tumor cell growth and cause progenotoxic effects.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.