Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Reactive Solute Transport in an Acidic Stream: Experimental pH Increase and Simulation of Controls on pH, Aluminum, and Iron
TL;DR: Solute transport simulations quantitatively constrained hydrologic and geochemical hypotheses about field observations of a pH modification in an acid mine drainage stream, and revealed limitations on assumptions that reac tions were controlled only by equilibrium chemistry as discussed by the authors.
Journal ArticleDOI
Squalene selectively protects mouse bone marrow progenitors against cisplatin and carboplatin-induced cytotoxicity in vivo without protecting tumor growth.
Bikul Das,Roula Antoon,Rika Tsuchida,Shamim Lotfi,Olena Morozova,Walid A. Farhat,David Malkin,Gideon Koren,Herman Yeger,Sylvain Baruchel +9 more
TL;DR: In vivo mouse model of cisplatin and cis-diammine (cyclobutane-1,1-dicarboxylato) platinum(II) (carboplatin)-induced toxicity is developed to further investigate squalene-mediated LD-BM cytoprotection including the molecular mechanism behind selective cy toprotection.
Journal ArticleDOI
Differential efficiency of induction of various lambdoid prophages responsible for production of Shiga toxins in response to different induction agents
TL;DR: It is found that efficiencies of induction of prophages and their further development vary considerably in response to different induction agents, and it is likely that pathogenicity of different STEC strains may be significantly different under specific conditions in their natural habitats.
Patent
Polysaccharide-bound nitric oxide-nucleophile adducts
TL;DR: A polymeric composition capable of releasing nitric oxide comprises a polysaccharide including a poly-releasing N2O2-functional group bound to the polymer as discussed by the authors.
Journal ArticleDOI
Mechanism of Honey Bacteriostatic Action Against MRSA and VRE Involves Hydroxyl Radicals Generated from Honey's Hydrogen Peroxide.
TL;DR: It is demonstrated for the first time that bacteriostatic effect of honeys on MRSA and VRE was dose-dependently related to generation of •OH from honey H2O2.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.