Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Mutations induced by ionizing radiation in a plasmid replicated in human cells. II. Sequence analysis of α-particle-induced point mutations
TL;DR: The data suggest that, although similar types of point mutations are induced in unirradiation, X-irradiated, and alpha-particle-ir radiated DNAs, the mechanisms of their induction and the exact nature of the lesions involved may be quite different.
Journal ArticleDOI
Natural antibacterial clays: historical uses and modern advances
TL;DR: A review of the historical uses of clays in medicine can be found in this paper, focusing primarily on the common characteristics of natural antibacterial clays and early studies of their antibacterial mechanisms.
Journal ArticleDOI
The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
Pablo Ivan Pereira Ramos,Márlon Grégori Flores Custódio,Guadalupe del Rosario Quispe Saji,Thiago Cardoso,Gisele Lucchetti da Silva,Graziela Braun,Willames M. B. S. Martins,Raquel Girardello,Ana Tereza Ribeiro de Vasconcelos,Elmer Andrés Fernández,Ana Cristina Gales,Marisa Fabiana Nicolás +11 more
TL;DR: The results supports the notion that the K. pneumoniae response to PB exposure goes beyond damaged membrane reconstruction and involves recruitment of multiple gene modules and intracellular targets.
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Inactivation of Escherichia coli by polychromatic simulated sunlight: evidence for and implications of a fenton mechanism involving iron, hydrogen peroxide, and superoxide.
Michael Fisher,Kara L. Nelson +1 more
TL;DR: Findings support the hypothesis that hydrogen peroxide, superoxide, and intracellular iron all participate in the photoinactivation of E. coli and suggest that the inactivation rate of enteric bacteria in the environment may be strongly dependent on iron availability and growth conditions.
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Oxidative modification of neurofilament-L by the Cu,Zn-superoxide dismutase and hydrogen peroxide system.
TL;DR: It is suggested that copper-mediated NF-L modification may be closely related to oxidative reactions which play a critical role in neurodegenerative diseases.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.