Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Endonucleases responsible for DNA repair of helix-distorting DNA lesions in the thermophilic crenarchaeon Sulfolobus acidocaldarius in vivo.
Shoji Suzuki,Norio Kurosawa +1 more
TL;DR: It is concluded that the endonuclease NucS/EndoMS works with XPF in homologous recombination-mediated stalled-fork DNA repair for the removal of helix-distorting DNA lesions in S. acidocaldarius.
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Synthesis and characterizations of natural limestone-derived nano-hydroxyapatite (HAp): a comparison study of different metals doped HAps on antibacterial activity
TL;DR: In this paper, different metal ions (i.e., Ag, Cu, Zn, and Mg) were doped into nano-hydroxyapatite (HAp) to improve the antibacterial activity.
Journal ArticleDOI
Biohybrid Cathode in Single Chamber Microbial Fuel Cell.
Giulia Massaglia,Giulia Massaglia,Isabella Fiorello,Isabella Fiorello,Adriano Sacco,Valentina Margaria,Candido Pirri,Candido Pirri,Marzia Quaglio +8 more
TL;DR: This study demonstrates that the highest power production is reached when aerobic biofilm acts as a catalyst for ORR in synergy with Pt in the biohybrid cathode.
Journal ArticleDOI
Current Use of Fenton Reaction in Drugs and Food
TL;DR: This review summarizes the recent growing interest in the usefulness of iron and its biological relevance through basic and practical information of the Fenton reaction and recent reports.
Journal ArticleDOI
A study of the ability of bioactive extracts from brewers' spent grain to enhance the antioxidant and immunomodulatory potential of food formulations following in vitro digestion
Aoife L. McCarthy,Yvonne C. O'Callaghan,Alan Connolly,Charles O. Piggott,Richard J. FitzGerald,Nora M. O'Brien +5 more
TL;DR: In this paper, the authors measured the bioactivity of a snack-bar, chocolate-drink and yogurt fortified with brewers' spent grain (BSG) phenolic extracts (P2 or B2) or protein hydrolysates (BPH), BPH Ã 0.5 and 0.1% (v/v) digestates for addition to Caco-2 and Jurkat T cells, respectively.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.