Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Classification of carcinogens: polemics, pedantics, or progress?
David B. Clayson,David J. Clegg +1 more
TL;DR: Mechanisms attributable to high dose toxicity occasioned by misuse of the maximum tolerated dose concept, imbalancing of homeostasis, unphysiological conditions, and induced cellular proliferation are reviewed.
Journal ArticleDOI
Hoechst-naphthalimide dyad with dual emissions as specific and ratiometric sensor for nucleus DNA damage
TL;DR: Hoe-NI is a recommendable sensor for the monitoring of nuclear DNA damage that reveals the health status of cells and provides the quantitative information in the processes of DNA damage induced by hydroxyl radicals and antitumor drug.
Journal ArticleDOI
Abiotic and biotic factors responsible for antimonite oxidation in Agrobacterium tumefaciens GW4
TL;DR: The data reveal a novel mechanism that bacterial Sb(III) oxidation is associated with abiotic (cellular H2O2) and biotic (AnoA and AioAB) factors and Sb’s oxidation process consumes cellular H2 O2 which contributes to microbial detoxification of both Sb (III) and cellular H 2O2.
Journal ArticleDOI
Chemical Warfare at the Microorganismal Level: A Closer Look at the Superoxide Dismutase Enzymes of Pathogens.
TL;DR: The chemistry of superoxide radicals and the role of diverse SOD metalloenzymes in bacterial, fungal, and protozoan pathogens are discussed and the unique features of microbial SOD enzymes that have evolved to accommodate the harsh lifestyle at the host-pathogen interface are highlighted.
Journal ArticleDOI
Potentiated homeopathic drug Arsenicum Album 30C inhibits intracellular reactive oxygen species generation and up-regulates expression of arsenic resistance gene in arsenite-exposed bacteria Escherichia coli.
Arnab Kumar De,Durba Das,Suman Dutta,Debrup Chakraborty,Naoual Boujedaini,Anisur Rahman Khuda-Bukhsh +5 more
TL;DR: Arsenicum Album 30C administration reduced arsenic toxicity in E. coli by inhibiting generation of ROS and increasing tolerance to arsenite toxicity and cell growth, validating efficacy of ultra-highly diluted remedies used in homeopathy.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.