Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
Reads0
Chats0
TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
More filters
Journal ArticleDOI
Polyamines protect Escherichia coli cells from the toxic effect of oxygen
TL;DR: Results show that polyamines are important in protecting cells from the toxic effects of oxygen, and it is reported that concentrations of H2O2 that are nontoxic to wild-type cells or to mutant cells pretreated with polyamines kill polyamine-deficient cells.
Journal ArticleDOI
Genetic toxicology of oxygen
TL;DR: Article de synthese sur la genotoxicite de l'oxygene et de ses metabolites et sur les mecanismes impliques
Journal ArticleDOI
Nanocatalysts-augmented Fenton chemical reaction for nanocatalytic tumor therapy
TL;DR: The rational design and fabrication of Fenton reaction-based nanocatalysts for triggering the in-situ Fenton chemical reaction within tumor microenvironment to generate highly toxic hydroxyl radicals (•OH), which is highly efficient for killing the cancer cells and suppressing the tumor growth is discussed.
Bactericidal Antibiotics Induce Toxic Metabolic Perturbations that Lead to Cellular Damage
Peter Belenky,Jonathan D. Ye,Nadia Cohen,Thomas C. Ferrante,Saloni R. Jain,Benjamin J. Korry,Eric G. Schwarz,Graham C. Walker,James J. Collins,Caroline B. M. Porter,Michael A. Lobritz +10 more
TL;DR: It is shown that bactericidal antibiotics induce a complex set of metabolic changes that are correlated with the buildup of toxic metabolic by-products, and potential end-target consequences of these metabolic perturbations are examined.
Journal ArticleDOI
Arsenic species that cause release of iron from ferritin and generation of activated oxygen.
TL;DR: Results indicate that exogenous methylated arsenic species and endogenous ascorbic acid can cause the release of iron from ferritin, the iron-dependent formation of reactive oxygen species, and DNA damage, which could be a mechanism of action of arsenic carcinogenesis in man.
References
More filters
Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.