Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Antimicrobial mechanism of alkyl gallates against Escherichia coli and Staphylococcus aureus and its combined effect with electrospun nanofibers on Chinese Taihu icefish preservation.
Yu-gang Shi,Run-run Zhang,Chen-min Zhu,Mingfeng Xu,Qing Gu,Rammile Ettelaie,Shan Lin,Yi-fan Wang,Xin-yi Leng +8 more
TL;DR: This research provides a more in-depth understanding of the interaction between OG and microorganisms and highlights the great promise of using OG as a safe multi-functionalized food additive for food preservations.
Journal ArticleDOI
Proteomic and transcriptomic characterization of a virulence-deficient phosphatidylcholine-negative Agrobacterium tumefaciens mutant.
Sonja Klüsener,Stephanie Hacker,Yun-Long Tsai,Julia E. Bandow,Ronald Gust,Erh-Min Lai,Franz Narberhaus +6 more
TL;DR: The integrated analysis revealed that A. tumefaciens dynamically remodels its membrane protein composition in order to sustain normal growth in the absence of PC, and validated the impaired virulence gene induction responsible for the virulence defect of the PC-deficient mutant.
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Solar photocatalytic disinfection of agricultural pathogenic fungi (Curvularia sp.) in real urban wastewater
TL;DR: The efficiency of solar AOPs are shown as a feasible option for the inactivation of resistant pathogens in water for crops irrigation, even in the presence of organic matter (average Dissolved Organic Carbon (DOC): 24mgL-1), and open a window for future wastewater reclamation and irrigation use.
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Mutation of potassium permanganate- and hydrogen peroxide-treated plasmid pZ189 replicating in CV-1 monkey kidney cells.
TL;DR: The data suggest that KMnO4-modified DNA is only weakly mutagenic in DNA replicating in mammalian nuclei, despite substantial production of Thy glycol and other base modifications, whereas H2O2/Fe(2+)-DTPA- modified DNA is more Mutagenic.
Journal Article
Expression of c-fos in Quiescent Swiss 3T3 Cells Exposed to Aqueous Cigarette Smoke Fractions
TL;DR: The data presented suggest that PP-1/2A may, at least in vitro, be targeted by water-soluble active compounds present in cigarette smoke, and other CS-dependent cellular and biochemical effects are similar to those induced in vitro by okadaic acid.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.