Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Journal ArticleDOI
DNA damage in barn swallows (Hirundo rustica) from the Chernobyl region detected by use of the comet assay.
Andrea Bonisoli-Alquati,Andrew Voris,Andrew Voris,Timothy A. Mousseau,Anders Pape Møller,Nicola Saino,Michael D. Wyatt +6 more
TL;DR: Levels of DNA damage, as indexed by the extent of DNA migration, were increased in barn swallows living in areas surrounding Chernobyl when compared to swallows sampled at low-level sites, indicating that chronic exposure to radioactive contaminants, even 20years after the accident at the Chernobyl nuclear power plant, continues to induce DNA damage in cells of free-living animals.
Journal ArticleDOI
Oxidizing intermediates generated in the Fenton reagent: kinetic arguments against the intermediacy of the hydroxyl radical.
TL;DR: The quenching procedure provides a unique spectrophotometric probe with which to examine the relative reactivities of putative Fenton-type oxidizing intermediates toward organic substrates, plus an overview of the mechanistic investigations of the Fenton reaction.
Book ChapterDOI
The role of iron coordination in the production of reactive oxidants from ferrous iron oxidation by oxygen and hydrogen peroxide
Journal ArticleDOI
Remediation of diesel-contaminated soil using in situ chemical oxidation (ISCO) and the effects of common oxidants on the indigenous microbial community: a comparison study
TL;DR: In this article, batch experiments were conducted using diesel-contaminated soil collected from a former gas station to evaluate the efficiency of diesel removal by in situ chemical oxidation (ISCO) and the effects of different oxidants on the indigenous microbial community.
Journal ArticleDOI
Effect of extract of medicinal plants on the labeling of blood elements with Technetium-99m and on the morphology of red blood cells: I—a study with Paullinia cupana
J.F de Oliveira,A.S Ávila,A. C.S. Braga,M. B. N. de Oliveira,Edson Boasquevisque,R.L. Jales .,Valbert Nascimento Cardoso,Mario Bernardo-Filho +7 more
TL;DR: In this article, Paullinia cupana is used in popular medicine and the authors evaluated its influence on the labeling process using technetium-99m (Tc99m).
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.