Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Antioxidative properties of Lactobacillus sake upon exposure to elevated oxygen concentrations
TL;DR: The antioxidative properties in Lactobacillus sake strains from different food origins were determined at low temperature and upon exposure to oxygen levels between 20 and 90% O(2), and it is concluded that its removal is essential for cell protection at elevated O( 2) conditions.
Journal ArticleDOI
Lack of the antioxidant glutathione peroxidase-1 does not increase atherosclerosis in C57BL/J6 mice fed a high-fat diet.
Judy B. de Haan,Judy B. de Haan,Paul K. Witting,Paul K. Witting,Nada Stefanovic,Josefa Pete,Michael Daskalakis,Ismail Kola,Roland Stocker,Joseph J Smolich +9 more
TL;DR: A specific deficiency in Gpx1 was not accompanied by an increase in markers of oxidative damage or increased atherosclerosis in a murine model of HFD-induced atherogenesis.
Journal ArticleDOI
Moderate wine consumption protects against hydrogen peroxide-induced DNA damage
TL;DR: The hypothesis that moderate wine drinking can protect somatic cells against the DNA-damaging effect of hydrogen peroxide is tested and it is supported by data from in vitro experiments showing that incubation of lymphocytes either with alcohol or wine stripped of phenolic compounds resulted in a statistically significant dose-related reduction in hydrogenperoxide-induced micronucleated cell frequency.
Journal ArticleDOI
Effects of inorganic selenium compounds on oxidative DNA damage
TL;DR: Electrophoresis experiments with [Fe(EDTA)](2-) (400 microM) and Na2SeO3 or SeO2 show that metal coordination by the selenium compound is required for antioxidant activity, and Na 2SeO4 may be safer than Na2 SeO3 for nutritional supplements.
Patent
Compounds and methods for the diagnosis, treatment and prevention of diseases of cell death
TL;DR: In this paper, the family of genes responsible for the neurodegenerative diseases, particularly Amyotrophic Lateral Sclerosis, were disclosed and methods and compounds for the diagnosis, prevention, and therapy of the disease were also disclosed.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.