Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Electrochemical and spectrometric study of antioxidant activity of pomiferin, isopomiferin, osajin and catalposide
Václav Diopan,Petr Babula,Violetta Shestivska,Vojtech Adam,Milan Zemlicka,Margita Dvorská,Jaromir Hubalek,Libuše Trnková,Ladislav Havel,René Kizek +9 more
TL;DR: Pomiferin had the highest antioxidant activity followed by isopomifer in, osajin and catalposide, and the activities of osaj in and catAlposide were undetectable.
Journal ArticleDOI
Identification of the Treponema pallidum subsp. pallidum TP0092 (RpoE) Regulon and Its Implications for Pathogen Persistence in the Host and Syphilis Pathogenesis
TL;DR: The results suggest that the TP0092 regulon is important in protecting the syphilis spirochete from damage caused by ROS produced at the site of infection during the inflammatory response.
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Hydrogen Peroxide Damage to Human Corneal Epithelial Cells In Vitro: Implications for Contact Lens Disinfection Systems
TL;DR: Hydrogen peroxide caused cell retraction as well as cessation of cell movement and mitotic activity, and users will be well served if the long-term effects of residual peroxide on the cornea are subjected to continued study.
Journal ArticleDOI
Curcumin and Other Polyphenolic Compounds in Head and Neck Cancer Chemoprevention
TL;DR: Cancer chemopreventive attempts with emphasis on head and neck carcinogenesis are summarized and some methodological issues are discussed, including data regarding antimutagenic effects of curcumin and epigallocatechin-3-gallate in human oropharyngeal mucosa cultures exposed to cigarette smoke condensate.
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Iron-dependent cleavage of ribosomal RNA during oxidative stress in the yeast Saccharomyces cerevisiae.
Jessica A. Zinskie,Arnab Ghosh,Brandon M Trainor,Daniel Shedlovskiy,Dimitri G. Pestov,Natalia Shcherbik +5 more
TL;DR: Evidence is provided that oxidative stress–associated rRNA cleavages can occur through rRNA strand scission by redox-active, ribosome-bound iron that potentially promotes Fenton reaction–induced hydroxyl radical production, implicating intracellular iron as a key determinant of the effects of oxidative stress on ribosomes.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.