Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
Reads0
Chats0
TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
More filters
Journal ArticleDOI
The mcsB gene of the clpC operon is required for stress tolerance and virulence in Staphylococcus aureus.
TL;DR: Data show that an S. aureus strain lacking functional mcsB is stress hypersensitive and therefore less viable when introduced into hostile environments, and for the first time, these studies have identified mCSB as a crucial and necessary component of stress and pathogenicity mechanisms.
Journal ArticleDOI
Heme sensing and detoxification by HatRT contributes to pathogenesis during Clostridium difficile infection.
Reece J. Knippel,Joseph P. Zackular,Jessica L. Moore,Arianna I. Celis,Andy Weiss,M. Kay Washington,Jennifer L. DuBois,Richard M. Caprioli,Eric P. Skaar +8 more
TL;DR: A mechanism employed by C. difficile to relieve heme toxicity within the host is described, and the stage for the development of therapeutic interventions to target this bacterial-specific system is set.
Journal ArticleDOI
Activities of antioxidant enzymes in three bacteria exposed to bensulfuron-methyl.
TL;DR: BSM could bring short-term ecotoxicity to three bacteria, but the effect of BSM was not lethal, and the SOD isoenzyme profiles of these bacteria were detected.
Journal ArticleDOI
Mechanism of metal-mediated DNA damage induced by metabolites of carcinogenic 2-nitropropane
TL;DR: It is suggested that metal-mediated DNA damage caused by 2-NP metabolites plays an important role in the mutagenicity and the carcinogenicity of 2- NP.
Journal ArticleDOI
Trade-Off between Iron Uptake and Protection against Oxidative Stress: Deletion of cueO Promotes Uropathogenic Escherichia coli Virulence in a Mouse Model of Urinary Tract Infection
Jai J. Tree,Glen C. Ulett,Cheryl-lynn Y. Ong,Darren J. Trott,Alastair G. McEwan,Mark A. Schembri +5 more
TL;DR: The deletion of cueO in uropathogenic Escherichia coli increases its colonization of the urinary tract despite its increased sensitivity to hydrogen peroxide, suggesting that the cueO deletion mutant accumulated iron with increased efficiency compared to its parent strain.
References
More filters
Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.