Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Aggregation of α-synuclein induced by the Cu, Zn-superoxide dismutase and hydrogen peroxide system
TL;DR: The results suggest that the Cu,Zn-SOD/H(2)O( 2) system might be related to abnormal aggregation of alpha-synuclein, which may be involved in the pathogenesis of PD and related disorders.
Journal ArticleDOI
Interactions of plasma-activated water with biofilms : inactivation, dispersal effects and mechanisms of action
Anne Mai-Prochnow,Renwu Zhou,Tianqi Zhang,Kostya Ostrikov,Sudarsan Mugunthan,Scott A. Rice,Scott A. Rice,Patrick J. Cullen +7 more
TL;DR: In this article, the formation of plasma-activated water (PAW) generated species and their impacts on biofilms are discussed. But the precise mode of action is still the subject of debate.
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Approach to Deliver Two Antioxidant Enzymes with Mesoporous Silica Nanoparticles into Cells
Yu Hsuan Lin,Yi Ping Chen,Tsang Pai Liu,Fan Ching Chien,Chih Ming Chou,Chien Tsu Chen,Chung-Yuan Mou +6 more
TL;DR: Simultaneous delivery of two up-downstream antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GPx) reveals synergistic efficiency of ROS scavenging, compared to single antioxidant enzyme delivery.
Production of Superoxide in Bacteria Is Stress- and Cell State-Dependent: A Gating-Optimized Flow Cytometry Method that Minimizes ROS Measurement Artifacts with Fluorescent Dyes
Megan E. McBee,Yok Hian Chionh,Mariam L. Sharaf,Peiying Ho,Maggie W. L. Cai,Maggie W. L. Cai,Peter C. Dedon +6 more
TL;DR: In this paper, the role of reactive oxygen species (ROS) in microbial metabolism and stress response has emerged as a major theme in microbiology and infectious disease and a robust platform for flow cytometric quantification of ROS in bacteria using fluorescent dyes, with ROS measurements in tens of thousands of individual cells under a variety of conditions.
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Oxidative injury mediated by the hepatic cytochrome P-450 system in conjunction with cellular iron. Effects on the pathway of haem biosynthesis
Andrew G. Smith,F De Matteis +1 more
TL;DR: These findings provide an experimental model for the human disease porphyria cutanea tarda and indicate that there may also be an association between the induction of uroporphyria and the development of liver tumours after administration of polyhalogenated aromatic chemicals.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.