Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Biochemical Basis for Functional Ingredient Design from Fruits
Jissy K. Jacob,Krishnaraj Tiwari,Julieta Correa-Betanzo,Azizah Misran,Renu Chandrasekaran,Gopinadhan Paliyath +5 more
TL;DR: Functional food ingredients (nutraceuticals) in fruits range from small molecular components, such as the secondary plant products, to macromolecular entities, e.g., pectin and cellulose, that provide several health benefits.
Journal ArticleDOI
Effects of Cr(VI) on the expression of the oxidative stress genes in human lung cells
TL;DR: The data obtained indicate that heme oxygenase, known to be a stress-inducible gene, may be involved in cellular pathways critical to the carcinogenic activity of Cr(VI) in normal human lung cells.
Journal ArticleDOI
Oxidative stress in E. coli cells upon exposure to heat treatments
TL;DR: Data presented in this work support the view that ROS generation and subsequent control in bacterial cells could be an essential factor determining inactivation and survival upon exposure to heat, and it could be a potential target to increase the efficacy of current treatments.
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Alleviative effect of quercetin on rat testis against arsenic: a histological and biochemical study
TL;DR: It was concluded that quercetin may be used as a potential therapeutic drug against arsenic induced reproductive toxicity in male Sprague Dawely rats.
Journal ArticleDOI
Dirty hands: photodynamic killing of human pathogens like EHEC, MRSA and Candida within seconds
Anja Eichner,Fernanda Pereira Gonzales,Ariane Felgenträger,Johannes Regensburger,Thomas Holzmann,Wulf Schneider-Brachert,Wolfgang Bäumler,Tim Maisch +7 more
TL;DR: TMPyP exhibited a fast and very effective killing rate of life-threatening pathogens within a couple of seconds that encourages further testing in an in vivo setting and antimicrobial photodynamic applications might become acceptable as a tool for hand hygiene procedures and also in other skin areas.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.