Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Book ChapterDOI
Structure, mechanism and physiological roles of bacterial cytochrome c peroxidases.
John M. Atack,David J. Kelly +1 more
TL;DR: The structure, mechanism and possible roles of bCCPs are examined in the context of their periplasmic location, the regulation of their synthesis by oxygen and their particular function in pathogens.
Journal Article
DNA Markers of Oxidative Processes in Vivo: Relevance to Carcinogenesis and Anticarcinogenesis
TL;DR: Although measurement of markers of oxidative stress relevant to carcinogenesis is at an early stage of development, this approach will probably become an integral part of early diagnostics and the assessment of tumor metabolism, particularly chemoprevention.
Journal ArticleDOI
Quantitative measurements of the generation of hydroxyl radicals by soot particles in a surrogate lung fluid
TL;DR: In this paper, a technique to quantitatively and specifically measure reactive oxygen species (ROS) in an aqueous, buffered extract solution as a surrogate lung fluid (SLF) was developed.
Journal ArticleDOI
Lethal synergy of solar UV-radiation and H2O2 on wild Fusarium solani spores in distilled and natural well water
TL;DR: This paper shows how H(2)O (2) disinfection concentrations could be significantly reduced by using the synergic lethality of H( 2)O(1) and sunlight the first time for fungi and disinfection.
Journal ArticleDOI
Experimental evidence that clay inhibits bacterial decomposers: Implications for preservation of organic fossils
TL;DR: It is shown that clays of particle size <2 μm in suspensions exceeding 10 mg/ml in concentration inhibit the growth of Pseudoalteromonas luteoviolacea, a marine heterotrophic bacterium involved in the decay of marine animals.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.