Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Glucose-Responsive Sequential Generation of Hydrogen Peroxide and Nitric Oxide for Synergistic Cancer Starving-Like/Gas Therapy.
Wenpei Fan,Wenpei Fan,Nan Lu,Peng Huang,Yi Liu,Zhen Yang,Sheng Wang,Guocan Yu,Yijing Liu,Junkai Hu,Qianjun He,Junle Qu,Tianfu Wang,Xiaoyuan Chen +13 more
TL;DR: A novel treatment paradigm of starving-like therapy is developed for significant tumor-killing effects, more effective than conventional starving therapy by only cutting off the energy supply by using hollow mesoporous organosilica nanoparticle as a biocompatible/biodegradable nanocarrier.
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ideR, an Essential Gene in Mycobacterium tuberculosis: Role of IdeR in Iron-Dependent Gene Expression, Iron Metabolism, and Oxidative Stress Response
TL;DR: Evidence is provided that ideR is an essential gene in Mycobacterium tuberculosis in the absence of a second functional copy of the gene and that the genes regulated by iron and IdeR were identified.
Journal ArticleDOI
Lethal oxidative damage and mutagenesis are generated by iron in delta fur mutants of Escherichia coli: protective role of superoxide dismutase.
TL;DR: It is proposed that a transient iron overload occurs in wild-type cells on return to normal growth conditions following iron starvation, with the coupling between iron and MnSOD regulation helping the cells cope.
Journal ArticleDOI
Protection of DNA during oxidative stress by the nonspecific DNA-binding protein Dps.
Asuncion Martinez,Roberto Kolter +1 more
TL;DR: In a purified in vitro system, Dps reduces the number of DNA single-strand breaks and Fpg-sensitive sites introduced by hydrogen peroxide treatment, indicating that the protection observed in vivo is a direct effect of DNA binding by Dps.
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Antimicrobial silver nanomaterials
TL;DR: This review describes the mode of action of silver nanoparticles (Ag NPs) in damaging the bacterial extracellular membrane and their intracellular components that allows them to exhibit wide spectrum antimicrobial effect and suggests possible strategies to tune these physicochemical properties to optimize their bactericidal properties.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.