Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Real-Time Multimodal Bioimaging of Cancer Cells and Exosomes through Biosynthesized Iridium and Iron Nanoclusters.
TL;DR: The facile in situ biosynthesis of iridium and iron oxide nanoclusters in cancer cells or tumor tissue is reported, demonstrating that highly luminescent and magnetic NCs are not only biocompatible but also tumor-targeted because NC formation does not take place in normal cells and tissues.
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The etiology of cancer: hydroxyl radical-induced DNA lesions in histologically normal livers offish from a population with liver tumors
Donald C. Malins,Russom Haimanot +1 more
TL;DR: With the exception of FapyAde, the DNA lesion concentrations in the livers were intermediate between those of the initially studied tumor-bearing fish and healthy fish from non-contaminated sites, which is consistent with the concept that they are causally linked to tumorigenesis.
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Azurin of Pathogenic Neisseria spp. Is Involved in Defense against Hydrogen Peroxide and Survival within Cervical Epithelial Cells
Hsing Ju Wu,Kate L. Seib,Jennifer L. Edwards,Michael A. Apicella,Alastair G. McEwan,Michael P. Jennings +5 more
TL;DR: Laz, a lipid-modified azurin of the human pathogens NeISSeria gonorrhoeae and Neisseria meningitidis, is involved in defense against oxidative stress and copper toxicity; laz mutant strains are hypersensitive to hydrogen peroxide and copper.
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Non-lethal exposure to H2O2 boosts bacterial survival and evolvability against oxidative stress.
Alexandro Rodríguez-Rojas,Joshua Jay Kim,Paul R. Johnston,Paul R. Johnston,Olga Makarova,Murat Eravci,Christoph Weise,Regine Hengge,Jens Rolff +8 more
TL;DR: It is found that a low level of H2O2 induced several scavenging enzymes showing a long half-life, subsequently protecting cells from future exposure, and experimental evolution of H 2O2 resistance revealed faster evolution and higher levels of resistance in primed cells.
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The effect of oxidants on biomembranes and cellular metabolism
Satish K. Srivastava,Naseem H. Ansari,Siqi Liu,Anja Izban,Ballabh Das,Gabor Szabo,Aruni Bhatnagar +6 more
TL;DR: By using patch-clamp technique in isolated frog myocytes, it is shown that hydroxy radicals generated by ferrous sulfate and ascorbate as well as lipid peroxides such as t-butyl hydroperoxide facilitate the entry of Na+ by oxidizing Na+-channels.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.