Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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ATM deficiency and oxidative stress: a new dimension of defective response to DNA damage.
TL;DR: This connection between genome instability-oxidative stress connection and ATM deficiency may provide new insights into the phenotypes associated with genetic deficiencies of DNA damage responses, and point to new strategies to alleviate some of their clinical symptoms.
Oxidation of the Guanine Nucleotide Pool Underlies Cell Death by Bactericidal Antibiotics
TL;DR: The efforts to understand why DinB (DNA polymerase IV) overproduction is cytotoxic to Escherichia coli led to the unexpected insight that oxidation of guanine to 8-oxo-guanine in the nucleotide pool underlies much of the cell death caused by both DinB overproduction and bactericidal antibiotics.
Journal ArticleDOI
Similarities and differences in the responses of microorganisms to biocides
TL;DR: Unlike antibiotics, biocides are multi-targeted antimicrobial agents and several of the damaging effects reported to occur in the most widely studied organisms, bacteria, may also take place to varying degrees in other organisms.
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Opposite base‐dependent reactions of a human base excision repair enzyme on DNA containing 7,8‐dihydro‐8‐oxoguanine and abasic sites
Magnar Bjørås,Luisa Luna,Barbro Johnsen,Elsebeth Hoff,Terje Haug,Torbjørn Rognes,Erling Seeberg +6 more
TL;DR: It appears that strand incisions are made only if repair completion results in correct base insertion, whereas excision from mispairs preserves strand continuity and hence allows for error‐free correction by a postreplicational repair mechanism.
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Antimicrobial properties of ZnO nanomaterials: A review
TL;DR: The recent developments for the fabrication of ZnO nanomaterials with variable morphologies, factors influencing the growth, morphology and surface defects, and various laboratory methods to evaluate the antibacterial activities toward Gram-positive as well as Gram-negative bacterial strains are reviewed.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.