Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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In situ self-assembled Ag–Fe3O4 nanoclusters in exosomes for cancer diagnosis
Tayyaba,Fawad Ur Rehman,Sana Shaikh,Tanziela,Farouk Semcheddine,Tianyu Du,Hui Jiang,Xuemei Wang +7 more
TL;DR: These findings demonstrate that the biocompatible nature of these self-assembled NCs loaded on exosomes could be utilized to bioimage cancer in the initial stages through fluorescence imaging.
Journal ArticleDOI
Hydrogen peroxide induces DNA single strand breaks in respiratory epithelial cells
TL;DR: DNA single strand break formation by alkaline elution was evaluated as a measure of oxidant-induced DNA damage to bronchial epithelial cells and BEAS cells were more sensitive to H2O2-induced injury than primary bronchials.
Journal ArticleDOI
Nanosilver and the microbiological activity of the particulate solids versus the leached soluble silver.
Merisa Bestari Faiz,Rose Amal,Christopher P. Marquis,Elizabeth J. Harry,Georgios A. Sotiriou,Scott A. Rice,Cindy Gunawan +6 more
TL;DR: It is shown that future enquiries on the antimicrobial potency and also importantly, the environmental and clinical impact of Ag NPs use, should pay attention to the potential bacterial toxicological responses to the undissolved Ag particulates, rather than just to the leaching of soluble silver.
Journal ArticleDOI
Interaction between Streptococcus pneumoniae and Staphylococcus aureus Generates ·OH Radicals That Rapidly Kill Staphylococcus aureus Strains.
Xueqing Wu,Xueqing Wu,Oren Gordon,Wenxin Jiang,Brenda S. Antezana,Uriel A. Angulo-Zamudio,Carlos del Rio,Abraham G. Moller,Terry Brissac,Aimee R. P. Tierney,Kurt Warncke,Carlos J. Orihuela,Timothy D. Read,Jorge E. Vidal +13 more
TL;DR: Spn produces H2O2 which is rapidly converted to a more potent oxidant, the hydroxyl radicals, to rapidly intoxicate Sau strains, and this discovery represents a new alternative for the development of antimicrobials against Sau infections.
Journal ArticleDOI
Evolution of Escherichia coli for maximum HOCl resistance through constitutive expression of the OxyR regulon.
Jasmin Gundlach,Jeannette Winter +1 more
TL;DR: Exposure of cells to stress impairs cellular functions and may cause killing or adaptation, and the question whether bacteria can adapt to high HOCl doses and if so, how the acquired resistance is facilitated is addressed.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.