Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Journal ArticleDOI
Copper starvation-inducible protein for cytochrome oxidase biogenesis in Bradyrhizobium japonicum.
Fabio Serventi,Zeb Andrew Youard,Valérie Murset,Simona G. Huwiler,Doris Bühler,Miriam Richter,Ronny Luchsinger,Hans-Martin Fischer,Robert Brogioli,Martina Niederer,Hauke Hennecke +10 more
TL;DR: Evidence is provided that PcuC and the copper chaperone ScoI are important for the symbiotically essential, CuA-free cbb3-type cytochrome oxidase specifically in endosymbiotic bacteroids of soybean root nodules, which could explain the symbiosis-defective phenotype of the pcu C and scoI mutants.
Book ChapterDOI
The Role of Intermetal Competition and Mis-Metalation in Metal Toxicity.
TL;DR: A simple model is introduced by which to understand bacterial metal homeostasis in terms of the distribution of each essential metal ion within cellular 'pools', and how these pools interact with each other and with key proteins of bacterial metalHomeostasis is dissected.
Journal ArticleDOI
Genotoxic and mutagenic effects of guarana (Paullinia cupana) in prokaryotic organisms
TL;DR: Aqueous extracts of Paullinia cupana (guarana), a species that belongs to the Sapindaceae family, were analyzed for the presence of genotoxic activities in bacterial cells, suggesting that oxygen reactive species play an essential role in the genotoxicity of aqueous guarana extracts.
Journal ArticleDOI
Increased hepcidin expression in non‐small cell lung cancer tissue and serum is associated with clinical stage
TL;DR: Hepcidin is a small secreted peptide that plays a key role in iron metabolism and may be implicated in colorectal cancer, but the relationship between hepcidin and lung cancer has not yet been studied.
Journal ArticleDOI
Rat enterocyte injury by oxygen-dependent processes.
Susan S. Baker,Cara L. Campbell +1 more
TL;DR: The data suggest that O2- and H2O2, whether created by stimulated neutrophils or an enzyme-generating system, are damaging to isolated enterocytes.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.