Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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The YaaA Protein of the Escherichia coli OxyR Regulon Lessens Hydrogen Peroxide Toxicity by Diminishing the Amount of Intracellular Unincorporated Iron
TL;DR: During periods of H( 2)O(2) stress the induction of YaaA is a critical device to suppress intracellular iron levels; it thereby attenuates the Fenton reaction and the DNA damage that would otherwise result.
Journal ArticleDOI
Urinary biomarkers and the rate of DNA damage in carcinogenesis and anticarcinogenesis.
TL;DR: Since oxidative processes and accumulation of their effects contribute to carcinogenesis, the proposed rate-of-damage hypothesis provides a rationale for using these biomarkers in early diagnostics and in the assessment of carcinogenic and anticarcinogenic properties of diets, foods, and food components, as well as certain exogenous toxicants and agents.
Journal ArticleDOI
Human adenoma cells are highly susceptible to the genotoxic action of 4-hydroxy-2-nonenal.
Anja Schaeferhenrich,Gabriele Beyer-Sehlmeyer,Grit Festag,A. Kuechler,Natja Haag,Anja Weise,Thomas Liehr,Uwe Claussen,Brigitte Marian,Wolfgang Sendt,J. Scheele,Beatrice L. Pool-Zobel +11 more
TL;DR: The LT97 is a relevant model for studying genotoxicity of colon cancer risk factors since colon adenoma are common preneoplastic lesions occurring in advanced age.
Journal ArticleDOI
Nfu facilitates the maturation of iron‐sulfur proteins and participates in virulence in Staphylococcus aureus
Ameya A. Mashruwala,Yun Y. Pang,Zuelay Rosario-Cruz,Harsimranjit K. Chahal,Meredith A. Benson,Laura A. Mike,Eric P. Skaar,Victor J. Torres,William M. Nauseef,Jeffrey M. Boyd +9 more
TL;DR: It is concluded that Nfu is necessary for staphylococcal pathogenesis and establish Fe‐S cluster metabolism as an attractive antimicrobial target.
Journal ArticleDOI
Inactivation of MS2 coliphage by Fenton's reagent.
TL;DR: Observations on the effects of pH and iron-chelating agents indicate that oxidants formed on the surface or inside MS2 are responsible for the inactivation.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.