Journal ArticleDOI
Toxic DNA damage by hydrogen peroxide through the Fenton reaction in vivo and in vitro.
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TLDR
An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide.Abstract:
Exposure of Escherichia coli to low concentrations of hydrogen peroxide results in DNA damage that causes mutagenesis and kills the bacteria, whereas higher concentrations of peroxide reduce the amount of such damage. Earlier studies indicated that the direct DNA oxidant is a derivative of hydrogen peroxide whose formation is dependent on cell metabolism. The generation of this oxidant depends on the availability of both reducing equivalents and an iron species, which together mediate a Fenton reaction in which ferrous iron reduces hydrogen peroxide to a reactive radical. An in vitro Fenton system was established that generates DNA strand breaks and inactivates bacteriophage and that also reproduces the suppression of DNA damage by high concentrations of peroxide. The direct DNA oxidant both in vivo and in this in vitro system exhibits reactivity unlike that of a free hydroxyl radical and may instead be a ferryl radical.read more
Citations
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Photoelectrochemical detection of oxidative DNA damage induced by Fenton reaction with low concentration and DNA-associated Fe2+.
TL;DR: It was found that Fe(2+) alone (without the coexistence of H(2)O(2)) suppressed the photocurrent of the intercalator bound to the DNA film in a pH-dependent manner, and Fe2+ ions associated with the DNA in the sensor film and participated in the DNA damage reaction, a mechanism that has been implicated in previous studies on metal carcinogenesis.
Journal Article
Selective neuronal differentiation of neural stem cells induced by nanosecond microplasma agitation
Zilan Xiong,Shasha Zhao,X. Mao,Xinpei Lu,Guangyuan He,G. Yang,Mingjie Chen,Musarat Ishaq,Ken Ostrikov +8 more
TL;DR: In this paper, atmospheric pressure room-temperature microplasma jets (MPJ) are used to selectively differentiate neural stem cells (NSCs) into neurons, which is a step towards reproducible and efficient production of the desired NSC derivatives.
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Glucose oxidase mediated targeted cancer-starving therapy by biotinylated self-assembled vesicles
TL;DR: Gl glucose oxidase mediated targeted cancer-starving therapy by self-assembled vesicle of trimesic acid based biotinylated amphiphile by blocking the energy supply to tumors through the oxidation of intracellular glucose.
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Differing effects of the inhibition of poly(ADP-ribose) polymerase on the course of oxidative cell injury in hepatocytes and fibroblasts.
TL;DR: The data indicate that the relationship between oxidative DNA damage and the genesis of lethal injury is very different in the two types of cells.
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Ascorbate oxidation reduction in Helicoverpa zea as a scavenging system against dietary oxidants
Gary W. Felton,Sean S. Duffey +1 more
TL;DR: There is circumstantial evidence for a protective mechanism utilizing ascorbate as an antioxidant and glutathione and/or NADPH as reductants in the midgut of larval Helicoverpa zea, and the particular relevance of this system to antioxidant protection is discussed.
References
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Journal ArticleDOI
The biology of oxygen radicals
TL;DR: The reactive superoxide radical, O2-, formerly of concern only to radiation chemists and radiobiologists, is now understood to be a normal product of the biological reduction of molecular oxygen.
Journal ArticleDOI
The catalytic decomposition of hydrogen peroxide by iron salts
Fritz Haber,Joseph Weiss +1 more
TL;DR: Wansbrough-Jones as discussed by the authors gave the manuscript of this paper to Professor Sir William Pope, but the final revision for the press had not been made and in its original from the paper was not suitable for publication in an English journal; but since, Professor Haber had considered carefully how he wished to present the results embodied in it, the form and sequence of the paper remain unmodified.