Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
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CRISPR-Cas9 based plant genome editing: Significance, opportunities and recent advances.
TL;DR: Recent advancements in CRISPR/Cas system and methods for analyzing the induced mutations, and its implementations in crop improvement are summarized.
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Current progress in CRISPR-based diagnostic platforms.
TL;DR: Integrating the CRISPR‐Cas platform along with lateral flow system allows rapid, sensitive, specific, cheap, and reliable diagnostic, which has the potential to be in frontline for pathogen detection during the epidemic outbreak.
Journal ArticleDOI
Application and future perspective of CRISPR/Cas9 genome editing in fruit crops
TL;DR: Challenges facing fruit crops as well as new improvements and platforms that could be used to facilitate genome editing in fruit crops are discussed, including dCas9-base-editing to introduce desirable alleles and heat treatment to increase editing efficiency.
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Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection
Kelsi Penewit,Elizabeth A. Holmes,Kathyrn McLean,Mingxin Ren,Adam Waalkes,Stephen J. Salipante +5 more
TL;DR: The development of a system for the facile and high-throughput genomic engineering of S. aureus using single-stranded DNA oligonucleotide recombineering coupled with clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9-mediated counterselection is reported.
Journal ArticleDOI
A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing
Peng Chen,Jin Zhou,Yibin Wan,Huan Liu,Yongzheng Li,Zhaoxin Liu,Hongjian Wang,Jun Lei,Kai Zhao,Yiliang Zhang,Yan Wang,Xinghua Zhang,Lei Yin +12 more
TL;DR: This study shows that CeCas12a nuclease is active in human cells and the stringency of PAM recognition could be an important factor shaping off-target editing in gene editing.
References
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Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
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Fast and accurate short read alignment with Burrows–Wheeler transform
Heng Li,Richard Durbin +1 more
TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
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MUSCLE: multiple sequence alignment with high accuracy and high throughput
TL;DR: MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.