Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
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Heat‐shock‐inducible CRISPR/Cas9 system generates heritable mutations in rice
TL;DR: HS‐CRISPR/Cas9 is a controlled and reasonably efficient platform for genome editing, and therefore, a promising tool for limiting genome‐wide off‐target effects and improving the precision of genome editing.
Journal ArticleDOI
Cpf1 enables fast and efficient genome editing in Aspergilli
TL;DR: The experiments demonstrate that Cpf1 can be efficiently used in Aspergilli for gene editing thereby expanding the range of genomic DNA sequences that can be targeted by CRISPR technologies.
Journal ArticleDOI
Gene Editing of Human Hematopoietic Stem and Progenitor Cells: Promise and Potential Hurdles
TL;DR: The specific applications of gene-editing technologies in human HSPCs, as informed by prior experience with gene addition strategies are discussed, focusing on strategies to move these techniques toward implementation in safe and effective clinical trials.
Patent
Crispr hybrid dna/rna polynucleotides and methods of use
TL;DR: The present disclosure provides DNA-guided CRISPR systems, polynucleotides comprising DNA, RNA and mixtures thereof, and methods of use involving such polynuclotides.
Journal ArticleDOI
CAMERS-B: CRISPR/Cpf1 assisted multiple-genes editing and regulation system for Bacillus subtilis.
TL;DR: A powerful tool called CRISPR/Cpf1 assisted multiple‐genes editing and regulation system for B. subtilis was constructed, a synthetic oligos mediated assembly ofCRISPR RNA (crRNA) array method was created, and the synthesis pathways of N‐acetylglucosamine and acetoin were engineered using this system.
References
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Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
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