Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TLDR
In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.About:
This article is published in Cell.The article was published on 2015-10-22 and is currently open access. It has received 3436 citations till now. The article focuses on the topics: CRISPR/Cpf1 & Cas9.read more
Citations
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Structural Basis for Guide RNA Processing and Seed-Dependent DNA Targeting by CRISPR-Cas12a.
TL;DR: Crystal structures of Francisella novicida Cas12a bound to guide RNA and in complex with an R-loop formed by a non-cleavable guide RNA precursor and a full-length target DNA reveal the mechanisms of guide RNA processing and pre-ordering of the seed sequence in the guide RNA that primes Cas 12a for target DNA binding and suggest a mechanism for double-stranded DNA cleavage involving a single active site.
Journal ArticleDOI
Efficient precise knockin with a double cut HDR donor after CRISPR/Cas9-mediated double-stranded DNA cleavage
Jian-Ping Zhang,Xiao-Lan Li,Guo-Hua Li,Wanqiu Chen,Cameron Arakaki,Gary D. Botimer,David J. Baylink,Lu Zhang,Wei Wen,Ya-Wen Fu,Jing Xu,Noah Chun,Weiping Yuan,Tao Cheng,Xiao-Bing Zhang +14 more
TL;DR: It is shown that a double cut HDR donor, which is flanked by single guide RNA (sgRNA)-PAM sequences and is released after CRISPR/Cas9 cleavage, increases HDR efficiency by twofold to fivefold relative to circular plasmid donors.
Journal ArticleDOI
A Genetic Variant Associated with Five Vascular Diseases Is a Distal Regulator of Endothelin-1 Gene Expression
Rajat M. Gupta,Joseph E. Hadaya,Aditi Trehan,Seyedeh M. Zekavat,Carolina Roselli,Derek Klarin,Connor A. Emdin,Catharina R.E. Hilvering,Valerio Bianchi,Christian Mueller,Amit Khera,Russell J.H. Ryan,Jesse M. Engreitz,Robbyn Issner,Noam Shoresh,Charles B. Epstein,Wouter de Laat,Jonathan D. Brown,Renate B. Schnabel,Bradley E. Bernstein,Sekar Kathiresan +20 more
TL;DR: CRISPR-edited stem cell-derived endothelial cells demonstrate rs9349379 regulates expression of endothelin 1 (EDN1), a gene located 600 kb upstream of PHACTR1, which may explain the pattern of risk for the five associated diseases.
Journal ArticleDOI
Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion
TL;DR: A new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9 is described, enabling A•T to G•C conversion at frequencies up to 7.5% in protoplasts and 59.1% in regenerated rice and wheat plants.
Journal ArticleDOI
Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing
Hao Yin,Chun-Qing Song,Sneha Suresh,Qiongqiong Wu,Stephen Walsh,Luke H. Rhym,Esther Mintzer,Mehmet Fatih Bolukbasi,Lihua Julie Zhu,Kevin J. Kauffman,Haiwei Mou,Alicia Oberholzer,Junmei Ding,Suet-Yan Kwan,Roman L. Bogorad,Timofei S. Zatsepin,Timofei S. Zatsepin,Victor Koteliansky,Scot A. Wolfe,Wen Xue,Robert Langer,Daniel G. Anderson +21 more
TL;DR: This work identifies regions of sgRNA that can be modified while maintaining or enhancing genome-editing activity, and develops an optimal set of chemical modifications for in vivo applications, and shows that a single intravenous injection into mice induces >80% editing of Pcsk9 in the liver.
References
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Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
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TL;DR: MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.